Succinate production from CO2-grown microalgal biomass as carbon source using engineered Corynebacterium glutamicum through consolidated bioprocessing

Jungseok Lee; Sang Jun Sim; Michael Bott; Youngsoon Um; Min Kyu Oh; Han Min Woo

doi:10.1038/srep05819

Succinate production from CO₂-grown microalgal biomass as carbon source using engineered Corynebacterium glutamicum through consolidated bioprocessing

Jungseok Lee, Sang Jun Sim, Michael Bott, Youngsoon Um, Min Kyu Oh, Han Min Woo

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

The potential for production of chemicals from microalgal biomass has been considered as an alternative route for CO₂ mitigation and establishment of biorefineries. This study presents the development of consolidated bioprocessing for succinate production from microalgal biomass using engineered Corynebacterium glutamicum. Starch-degrading and succinate-producing C. glutamicum strains produced succinate (0.16g succinate/g total carbon source) from a mixture of starch and glucose as a model microalgal biomass. Subsequently, the engineered C. glutamicum strains were able to produce succinate (0.28 g succinate/g of total sugars including starch) from pretreated microalgal biomass of CO₂ -grown Chlamydomonas reinhardtii. For the first time, this work shows succinate production from CO₂ via sequential fermentations of CO₂ -grown microalgae and engineered C. glutamicum. Therefore, consolidated bioprocessing based on microalgal biomass could be useful to promote variety of biorefineries.

Original language	English
Article number	5819
Journal	Scientific reports
Volume	4
DOIs	https://doi.org/10.1038/srep05819
Publication status	Published - 2014 Jul 24

Bibliographical note

Funding Information:
Authors thank Dr. Kyoungseon Min and Mr. Min Eui Hong for technical support. This work was supported by Korea CCS R&D Center (KCRC) (no. 2014M1A8A1049277) and by the National Research Foundation of Korea grant-funded by the Korean Government (Ministry of Science, ICT & Future Planning) (2014, University-Institute cooperation program).

ASJC Scopus subject areas

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10.1038/srep05819

Cite this

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title = "Succinate production from CO2-grown microalgal biomass as carbon source using engineered Corynebacterium glutamicum through consolidated bioprocessing",

abstract = "The potential for production of chemicals from microalgal biomass has been considered as an alternative route for CO2 mitigation and establishment of biorefineries. This study presents the development of consolidated bioprocessing for succinate production from microalgal biomass using engineered Corynebacterium glutamicum. Starch-degrading and succinate-producing C. glutamicum strains produced succinate (0.16g succinate/g total carbon source) from a mixture of starch and glucose as a model microalgal biomass. Subsequently, the engineered C. glutamicum strains were able to produce succinate (0.28 g succinate/g of total sugars including starch) from pretreated microalgal biomass of CO2 -grown Chlamydomonas reinhardtii. For the first time, this work shows succinate production from CO2 via sequential fermentations of CO2 -grown microalgae and engineered C. glutamicum. Therefore, consolidated bioprocessing based on microalgal biomass could be useful to promote variety of biorefineries.",

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note = "Funding Information: Authors thank Dr. Kyoungseon Min and Mr. Min Eui Hong for technical support. This work was supported by Korea CCS R&D Center (KCRC) (no. 2014M1A8A1049277) and by the National Research Foundation of Korea grant-funded by the Korean Government (Ministry of Science, ICT & Future Planning) (2014, University-Institute cooperation program).",

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AU - Oh, Min Kyu

AU - Woo, Han Min

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AB - The potential for production of chemicals from microalgal biomass has been considered as an alternative route for CO2 mitigation and establishment of biorefineries. This study presents the development of consolidated bioprocessing for succinate production from microalgal biomass using engineered Corynebacterium glutamicum. Starch-degrading and succinate-producing C. glutamicum strains produced succinate (0.16g succinate/g total carbon source) from a mixture of starch and glucose as a model microalgal biomass. Subsequently, the engineered C. glutamicum strains were able to produce succinate (0.28 g succinate/g of total sugars including starch) from pretreated microalgal biomass of CO2 -grown Chlamydomonas reinhardtii. For the first time, this work shows succinate production from CO2 via sequential fermentations of CO2 -grown microalgae and engineered C. glutamicum. Therefore, consolidated bioprocessing based on microalgal biomass could be useful to promote variety of biorefineries.

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