Sulfate assimilation regulates hydrogen sulfide production independent of lifespan and reactive oxygen species under methionine restriction condition in yeast

Kyung Mi Choi, Sorah Kim, Seahyun Kim, Hae Min Lee, Alaattin Kaya, Bok Hwan Chun, Yong Kwon Lee, Tae Sik Park, Cheol Koo Lee, Seong Il Eyun, Byung Cheon Lee

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Endogenously produced hydrogen sulfide was proposed to be an underlying mechanism of lifespan extension via methionine restriction. However, hydrogen sulfide regulation and its beneficial effects via methionine restriction remain elusive. Here, we identified the genes required to increase hydrogen sulfide production under methionine restriction condition using genome-wide high-throughput screening in yeast strains with single-gene deletions. Sulfate assimilation-related genes, such as MET1, MET3, MET5, and MET10, were found to be particularly crucial for hydrogen sulfide production. Interestingly, methionine restriction failed to increase hydrogen sulfide production in mutant strains; however, it successfully extended chronological lifespan and reduced reactive oxygen species levels. Altogether, our observations suggested that increased hydrogen sulfide production via methionine restriction is not the mechanism underlying extended yeast lifespan, even though increased hydrogen sulfide production occurred simultaneously with yeast lifespan extension under methionine restriction condition.

Original languageEnglish
Pages (from-to)4254-4273
Number of pages20
JournalAging
Volume11
Issue number12
DOIs
Publication statusPublished - 2019

Bibliographical note

Funding Information:
We thank Vadim N. Gladyshev (Harvard Medical School) and Christopher Hine (Harvard School of Public Health) for their valuable discussions and sharing their reagents. This work was supported by National Research Foundation of Korea (NRF) grants (2018R1A1A1 A05079386, 2018M3A9F3055925) funded by the Korean government (Ministry of Science, ICT & Future Planning) and the Korea University Future Research Grant awarded to B.C.L.

Publisher Copyright:
© Choi et al.

Keywords

  • High-throughput genetic screening
  • Hydrogen sulfide
  • Methionine restriction
  • Reactive oxygen species
  • Sulfate assimilation

ASJC Scopus subject areas

  • Ageing
  • Cell Biology

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