Abstract
Endogenously produced hydrogen sulfide was proposed to be an underlying mechanism of lifespan extension via methionine restriction. However, hydrogen sulfide regulation and its beneficial effects via methionine restriction remain elusive. Here, we identified the genes required to increase hydrogen sulfide production under methionine restriction condition using genome-wide high-throughput screening in yeast strains with single-gene deletions. Sulfate assimilation-related genes, such as MET1, MET3, MET5, and MET10, were found to be particularly crucial for hydrogen sulfide production. Interestingly, methionine restriction failed to increase hydrogen sulfide production in mutant strains; however, it successfully extended chronological lifespan and reduced reactive oxygen species levels. Altogether, our observations suggested that increased hydrogen sulfide production via methionine restriction is not the mechanism underlying extended yeast lifespan, even though increased hydrogen sulfide production occurred simultaneously with yeast lifespan extension under methionine restriction condition.
Original language | English |
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Pages (from-to) | 4254-4273 |
Number of pages | 20 |
Journal | Aging |
Volume | 11 |
Issue number | 12 |
DOIs | |
Publication status | Published - 2019 |
Bibliographical note
Funding Information:We thank Vadim N. Gladyshev (Harvard Medical School) and Christopher Hine (Harvard School of Public Health) for their valuable discussions and sharing their reagents. This work was supported by National Research Foundation of Korea (NRF) grants (2018R1A1A1 A05079386, 2018M3A9F3055925) funded by the Korean government (Ministry of Science, ICT & Future Planning) and the Korea University Future Research Grant awarded to B.C.L.
Publisher Copyright:
© Choi et al.
Keywords
- High-throughput genetic screening
- Hydrogen sulfide
- Methionine restriction
- Reactive oxygen species
- Sulfate assimilation
ASJC Scopus subject areas
- Ageing
- Cell Biology