Super-resolution fluorescent materials: An insight into design and bioimaging applications

Zhigang Yang, Amit Sharma, Jing Qi, Xiao Peng, Dong Yeop Lee, Rui Hu, Danying Lin, Junle Qu, Jong Seung Kim

Research output: Contribution to journalReview articlepeer-review

188 Citations (Scopus)


Living organisms are generally composed of complex cellular processes which persist only within their native environments. To enhance our understanding of the biological processes lying within complex milieus, various techniques have been developed. Specifically, the emergence of super-resolution microscopy has generated a renaissance in cell biology by redefining the existing dogma towards nanoscale cell dynamics, single synaptic vesicles, and other complex bioprocesses by overcoming the diffraction-imposed resolution barrier that is associated with conventional microscopy techniques. Besides the typical technical reliance on the optical framework and computational algorithm, super-resolution imaging microscopy resorts largely to fluorescent materials with special photophysical properties, including fluorescent proteins, organic fluorophores and nanomaterials. In this tutorial review article, with the emphasis on cell biology, we summarize the recent developments in fluorescent materials being utilized in various super-resolution techniques with successful integration into bio-imaging applications. Fluorescent proteins (FP) applied in super-resolution microscopy will not be covered herein as it has already been well summarized; additionally, we demonstrate the breadth of opportunities offered from a future perspective.

Original languageEnglish
Pages (from-to)4651-4667
Number of pages17
JournalChemical Society Reviews
Issue number17
Publication statusPublished - 2016 Sept 7

Bibliographical note

Funding Information:
This work is partially supported by the National Basic Research Program of China (No. 2015CB352005); the National Natural Science Foundation of China (No. 61525503/61378091/21406125); Guangdong Natural Science Foundation Innovation Team (No. 2014A030312008); Hong Kong, Macao and Taiwan cooperation innovation platform & major projects of international cooperation in Colleges and Universities in Guangdong Province (No. 2015KGJHZ002); and the Basic Science Research Program (No. 20100020209) project grants from the National Research Foundation of Korea.

Publisher Copyright:
© 2016 The Royal Society of Chemistry.

ASJC Scopus subject areas

  • General Chemistry


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