Surface Functionalization of Enzyme-Coronated Gold Nanoparticles with an Erythrocyte Membrane for Highly Selective Glucose Assays

Jae Won Jang, Hyunji Kim, Insu Kim, Sang Won Lee, Hyo Gi Jung, Kyo Seon Hwang, Jeong Hoon Lee, Gyudo Lee, Dongtak Lee, Dae Sung Yoon

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4 Citations (Scopus)


Colorimetric glucose sensors using enzyme-coronated gold nanoparticles have been developed for high-throughput assays to monitor the blood glucose levels of diabetic patients. Although those sensors have shown sensitivity and wide linear detection ranges, they suffer from poor selectivity and stability in detecting blood glucose, which has limited their practical use. To address this limitation, herein, we functionalized glucose-oxidase-coronated gold nanoparticles with an erythrocyte membrane (EM-GOx-GNPs). Because the erythrocyte membrane (EM) selectively facilitates the permeation of glucose via glucose transporter-1 (GLUT1), the functionalization of GOx-GNPs with EM improved the stability, selectivity (3.3- to 15.8-fold higher), and limit of detection (LOD). Both membrane proteins, GLUT1 and aquaporin-1 (AQP1), on EM were shown to be key components for selective glucose detection by treatment with their inhibitors. Moreover, we demonstrated the stability of EM-GOx-GNPs in high-antioxidant-concentration conditions, under long-term storage (∼4 weeks) and a freeze-thaw cycle. Selectivity of the EM-GOx-GNPs against other saccharides was increased, which improved the LOD in phosphate-buffered saline and human serum. Our results indicated that the functionalization of colorimetric glucose sensors with EM is beneficial for improving selectivity and stability, which may make them candidates for use in a practical glucose sensor.

Original languageEnglish
Pages (from-to)6473-6481
Number of pages9
JournalAnalytical chemistry
Issue number17
Publication statusPublished - 2022 May 3

Bibliographical note

Funding Information:
This work was supported by a National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (No. NRF-2018M3C1B7020722, NRF-2019R1A2B5B01070617, and NRF-2020R1A6A3A01096477). This research was also supported by a Korea University Graduate School Junior Fellow Research Grant. This work was supported by a Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare, the Ministry of Food and Drug Safety) (Project Number: 202012D19). This study was also supported by the BK21 FOUR (Fostering Outstanding Universities for Research). This work was also supported from Hyundai Motor Chung Mong-Koo Foundation.

Publisher Copyright:
© 2022 American Chemical Society. All rights reserved.

ASJC Scopus subject areas

  • Analytical Chemistry


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