TY - JOUR
T1 - Switchable stimulated Raman scattering microscopy with photochromic vibrational probes
AU - Ao, Jianpeng
AU - Fang, Xiaofeng
AU - Miao, Xianchong
AU - Ling, Jiwei
AU - Kang, Hyunchul
AU - Park, Sungnam
AU - Wu, Changfeng
AU - Ji, Minbiao
N1 - Funding Information:
M.J. acknowledges financial support from the National Nature Science Foundation of China (61975033), Shanghai Municipal Science and Technology Major Project (2017SHZDZX01, 2018SHZDZX01) and ZJLab, and Specialized Research Project of the Shanghai Health and Family Planning Commission on Smart Medicine (2018ZHYL0204). C.W. acknowledges financial support from Shenzhen Science and Technology Innovation Commission (KQTD20170810111314625), the National Natural Science Foundation of China (81771930), and the National Key Research and Development Program of China (2018YFB0407200). S.P. acknowledges financial support from the National Research Foundation of Korea (2019-R1A6A1A11044070).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12/1
Y1 - 2021/12/1
N2 - Photochromic probes with reversible fluorescence have revolutionized the fields of single molecule spectroscopy and super-resolution microscopy, but lack sufficient chemical specificity. In contrast, Raman probes with stimulated Raman scattering (SRS) microscopy provides superb chemical resolution for super-multiplexed imaging, but are relatively inert. Here we report vibrational photochromism by engineering alkyne tagged diarylethene to realize photo-switchable SRS imaging. The narrow Raman peak of the alkyne group shifts reversibly upon photoisomerization of the conjugated diarylethene when irradiated by ultraviolet (UV) or visible light, yielding “on” or “off” SRS images taken at the photoactive Raman frequency. We demonstrated photo-rewritable patterning and encryption on thin films, painting/erasing of cells with labelled alkyne-diarylethene, as well as pulse-chase experiments of mitochondria diffusion in living cells. The design principle provides potentials for super-resolution microscopy, optical memories and switches with vibrational specificity.
AB - Photochromic probes with reversible fluorescence have revolutionized the fields of single molecule spectroscopy and super-resolution microscopy, but lack sufficient chemical specificity. In contrast, Raman probes with stimulated Raman scattering (SRS) microscopy provides superb chemical resolution for super-multiplexed imaging, but are relatively inert. Here we report vibrational photochromism by engineering alkyne tagged diarylethene to realize photo-switchable SRS imaging. The narrow Raman peak of the alkyne group shifts reversibly upon photoisomerization of the conjugated diarylethene when irradiated by ultraviolet (UV) or visible light, yielding “on” or “off” SRS images taken at the photoactive Raman frequency. We demonstrated photo-rewritable patterning and encryption on thin films, painting/erasing of cells with labelled alkyne-diarylethene, as well as pulse-chase experiments of mitochondria diffusion in living cells. The design principle provides potentials for super-resolution microscopy, optical memories and switches with vibrational specificity.
UR - http://www.scopus.com/inward/record.url?scp=85106760594&partnerID=8YFLogxK
U2 - 10.1038/s41467-021-23407-2
DO - 10.1038/s41467-021-23407-2
M3 - Article
C2 - 34035304
AN - SCOPUS:85106760594
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 3089
ER -