Synergism of an auxiliary activity 9 (AA9) from Chaetomium globosum with xylanase on the hydrolysis of xylan and lignocellulose

In Jung Kim; Hak Jin Youn; Kyoung Heon Kim

doi:10.1016/j.procbio.2016.06.017

Synergism of an auxiliary activity 9 (AA9) from Chaetomium globosum with xylanase on the hydrolysis of xylan and lignocellulose

In Jung Kim, Hak Jin Youn, Kyoung Heon Kim

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Auxiliary activity family 9 (AA9) is a group of fungal synergistic proteins belonging to the lytic polysaccharide monooxygenases. Via oxidative cleavage, AA9 facilitates cellulose hydrolysis by cellulase. However, oxidative activity of AA9 on pure xylan has not been reported yet. Previously, an AA9 from Chaetomium globosum (CgAA9) was identified and expressed in Escherichia coli. The recombinant CgAA9 exhibited synergistic effects in cellulose hydrolysis. Here, we showed that CgAA9 acts synergistically in xylan hydrolysis. When the insoluble fraction of xylan from beechwood was hydrolyzed by xylanase, CgAA9 yielded 1.3 times more reducing sugar than that produced only by xylanase, and the extent of synergism by CgAA9 was enhanced in the presence of ascorbic acid as a reducing cofactor. In addition, CgAA9 functioned on pretreated rice straws. This is the first report of the synergistic activity of AA9 with xylanase in the hydrolysis of pure xylan and xylan contained in pretreated lignocellulose.

Original language	English
Pages (from-to)	1445-1451
Number of pages	7
Journal	Process Biochemistry
Volume	51
Issue number	10
DOIs	https://doi.org/10.1016/j.procbio.2016.06.017
Publication status	Published - 2016 Oct 1

Bibliographical note

Funding Information:
We want to acknowledge grant support from the Ministry of Trade, Industry & Energy ( 10049674 ) and facility support at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. IJK is grateful for a grant from Korea University .

Publisher Copyright:
© 2016 Elsevier Ltd

Keywords

Auxiliary activity 9
Chaetomium globosum
Lytic polysaccharide monooxygenase
Synergism
Xylan

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.procbio.2016.06.017

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title = "Synergism of an auxiliary activity 9 (AA9) from Chaetomium globosum with xylanase on the hydrolysis of xylan and lignocellulose",

abstract = "Auxiliary activity family 9 (AA9) is a group of fungal synergistic proteins belonging to the lytic polysaccharide monooxygenases. Via oxidative cleavage, AA9 facilitates cellulose hydrolysis by cellulase. However, oxidative activity of AA9 on pure xylan has not been reported yet. Previously, an AA9 from Chaetomium globosum (CgAA9) was identified and expressed in Escherichia coli. The recombinant CgAA9 exhibited synergistic effects in cellulose hydrolysis. Here, we showed that CgAA9 acts synergistically in xylan hydrolysis. When the insoluble fraction of xylan from beechwood was hydrolyzed by xylanase, CgAA9 yielded 1.3 times more reducing sugar than that produced only by xylanase, and the extent of synergism by CgAA9 was enhanced in the presence of ascorbic acid as a reducing cofactor. In addition, CgAA9 functioned on pretreated rice straws. This is the first report of the synergistic activity of AA9 with xylanase in the hydrolysis of pure xylan and xylan contained in pretreated lignocellulose.",

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note = "Funding Information: We want to acknowledge grant support from the Ministry of Trade, Industry & Energy ( 10049674 ) and facility support at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. IJK is grateful for a grant from Korea University . Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd",

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N1 - Funding Information: We want to acknowledge grant support from the Ministry of Trade, Industry & Energy ( 10049674 ) and facility support at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety. IJK is grateful for a grant from Korea University . Publisher Copyright: © 2016 Elsevier Ltd

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N2 - Auxiliary activity family 9 (AA9) is a group of fungal synergistic proteins belonging to the lytic polysaccharide monooxygenases. Via oxidative cleavage, AA9 facilitates cellulose hydrolysis by cellulase. However, oxidative activity of AA9 on pure xylan has not been reported yet. Previously, an AA9 from Chaetomium globosum (CgAA9) was identified and expressed in Escherichia coli. The recombinant CgAA9 exhibited synergistic effects in cellulose hydrolysis. Here, we showed that CgAA9 acts synergistically in xylan hydrolysis. When the insoluble fraction of xylan from beechwood was hydrolyzed by xylanase, CgAA9 yielded 1.3 times more reducing sugar than that produced only by xylanase, and the extent of synergism by CgAA9 was enhanced in the presence of ascorbic acid as a reducing cofactor. In addition, CgAA9 functioned on pretreated rice straws. This is the first report of the synergistic activity of AA9 with xylanase in the hydrolysis of pure xylan and xylan contained in pretreated lignocellulose.

AB - Auxiliary activity family 9 (AA9) is a group of fungal synergistic proteins belonging to the lytic polysaccharide monooxygenases. Via oxidative cleavage, AA9 facilitates cellulose hydrolysis by cellulase. However, oxidative activity of AA9 on pure xylan has not been reported yet. Previously, an AA9 from Chaetomium globosum (CgAA9) was identified and expressed in Escherichia coli. The recombinant CgAA9 exhibited synergistic effects in cellulose hydrolysis. Here, we showed that CgAA9 acts synergistically in xylan hydrolysis. When the insoluble fraction of xylan from beechwood was hydrolyzed by xylanase, CgAA9 yielded 1.3 times more reducing sugar than that produced only by xylanase, and the extent of synergism by CgAA9 was enhanced in the presence of ascorbic acid as a reducing cofactor. In addition, CgAA9 functioned on pretreated rice straws. This is the first report of the synergistic activity of AA9 with xylanase in the hydrolysis of pure xylan and xylan contained in pretreated lignocellulose.

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Synergism of an auxiliary activity 9 (AA9) from Chaetomium globosum with xylanase on the hydrolysis of xylan and lignocellulose

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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