TY - JOUR
T1 - Synergistic antimicrobial activity of metabolites produced by a nonobligate bacterial predator
AU - Cain, Cody C.
AU - Lee, Dongho
AU - Waldo, Robert H.
AU - Henry, Alexis T.
AU - Casida, Earl J.
AU - Wani, Mansukh C.
AU - Wall, Monroe E.
AU - Oberlies, Nicholas H.
AU - Falkinham, Joseph O.
PY - 2003/7/1
Y1 - 2003/7/1
N2 - A naturally occurring, gram-negative, nonobligate predator bacterial strain 679-2, exhibits broad-spectrum antimicrobial activity that is due, in part, to the production of three extracellular compounds. Antimicrobial-activity-directed fractionation of a culture of strain 679-2 against a panel of microorganisms has led to the isolation of three compounds: pyrrolnitrin, maculosin, and a new compound, which we have named banegasine. Although pyrroinitrin is well known in the literature, it has not been found in cells with the herbicide maculosin. Further, this is the first report of production of maculosin by a prokaryote. Both maculosin and banegasine, which displayed no antimicrobial activities alone, were found to potentiate the antimicrobial activity of pyrroinitrin. Based on 16S rRNA sequence, cellular fatty acid composition, and biochemical and cultural characteristics, strain 679-2 appears to represent a new genus and species of eubacteria, Aristabacter necator. The potent, broad-spectrum antimicrobial activity of predator strain 679-2 may be due to synergism between metabolites.
AB - A naturally occurring, gram-negative, nonobligate predator bacterial strain 679-2, exhibits broad-spectrum antimicrobial activity that is due, in part, to the production of three extracellular compounds. Antimicrobial-activity-directed fractionation of a culture of strain 679-2 against a panel of microorganisms has led to the isolation of three compounds: pyrrolnitrin, maculosin, and a new compound, which we have named banegasine. Although pyrroinitrin is well known in the literature, it has not been found in cells with the herbicide maculosin. Further, this is the first report of production of maculosin by a prokaryote. Both maculosin and banegasine, which displayed no antimicrobial activities alone, were found to potentiate the antimicrobial activity of pyrroinitrin. Based on 16S rRNA sequence, cellular fatty acid composition, and biochemical and cultural characteristics, strain 679-2 appears to represent a new genus and species of eubacteria, Aristabacter necator. The potent, broad-spectrum antimicrobial activity of predator strain 679-2 may be due to synergism between metabolites.
UR - http://www.scopus.com/inward/record.url?scp=0038000517&partnerID=8YFLogxK
U2 - 10.1128/AAC.47.7.2113-2117.2003
DO - 10.1128/AAC.47.7.2113-2117.2003
M3 - Article
C2 - 12821455
AN - SCOPUS:0038000517
SN - 0066-4804
VL - 47
SP - 2113
EP - 2117
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 7
ER -