Synergistic proteins for the enhanced enzymatic hydrolysis of cellulose by cellulase

In Jung Kim, Hee Jin Lee, In Geol Choi, Kyoung Heon Kim

    Research output: Contribution to journalReview articlepeer-review

    101 Citations (Scopus)

    Abstract

    Reducing the enzyme loadings for enzymatic saccharification of lignocellulose is required for economically feasible production of biofuels and biochemicals. One strategy is addition of small amounts of synergistic proteins to cellulase mixtures. Synergistic proteins increase the activity of cellulase without causing significant hydrolysis of cellulose. Synergistic proteins exert their activity by inducing structural modifications in cellulose. Recently, synergistic proteins from various biological sources, including bacteria, fungi, and plants, were identified based on genomic data, and their synergistic activities were investigated. Currently, an up-to-date overview of several aspects of synergistic proteins, such as their functions, action mechanisms and synergistic activity, are important for future industrial application. In this review, we summarize the current state of research on four synergistic proteins: carbohydrate-binding modules, plant expansins, expansin-like proteins, and Auxiliary Activity family 9 (formerly GH61) proteins. This review provides critical information to aid in promoting research on the development of efficient and industrially feasible synergistic proteins.

    Original languageEnglish
    Pages (from-to)8469-8480
    Number of pages12
    JournalApplied Microbiology and Biotechnology
    Volume98
    Issue number20
    DOIs
    Publication statusPublished - 2014 Oct

    Bibliographical note

    Publisher Copyright:
    © 2014, Springer-Verlag Berlin Heidelberg.

    Keywords

    • Auxiliary Activity family 9
    • Carbohydrate-binding module
    • Cellulase
    • Cellulose hydrolysis
    • Expansin
    • GH61
    • Synergistic protein

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

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