Synergistically enhanced osteoconductivity and anti-inflammation of PLGA/β-TCP/Mg(OH)2 composite for orthopedic applications

Seul Ki Lee; Cheol Min Han; Wooram Park; Ik Hwan Kim; Yoon Ki Joung; Dong Keun Han

doi:10.1016/j.msec.2018.09.011

Synergistically enhanced osteoconductivity and anti-inflammation of PLGA/β-TCP/Mg(OH)₂ composite for orthopedic applications

Seul Ki Lee, Cheol Min Han, Wooram Park, Ik Hwan Kim, Yoon Ki Joung, Dong Keun Han

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Synthetic biodegradable polymers including poly(lactide-co-glycolide) (PLGA) have been widely used as alternatives to metallic implantable materials in the orthopedic field due to their superior biocompatibility and biodegradability. However, weak mechanical properties of the biodegradable polymers and inflammatory reaction caused by the acidic degradation products have limited their biomedical applications. In this study, we have developed a PLGA composite containing beta-tricalcium phosphate (β-TCP) and magnesium hydroxide (Mg(OH)₂) as additives to improve mechanical, osteoconductivity, and anti-inflammation property of the biopolymer composite simultaneously. The β-TCP has an osteoconductive effect and the Mg(OH)₂ has a pH neutralizing effect. The PLGA/inorganic composites were uniformly blended via a twin extrusion process. The mechanical property of the PLGA/β-TCP/Mg(OH)₂ composite was improved compared to the pure PLGA. In particular, the addition of Mg(OH)₂ suppressed the inflammatory reaction of normal human osteoblast (NHOst) cells and also inhibited the differentiation of pre-osteoclastic cells into osteoclasts. Moreover, synergistically upregulated late osteogenic differentiation of NHOst cells was observed on the PLGA/β-TCP/Mg(OH)₂ composite. Taken all together, we believe that the use of β-TCP and Mg(OH)₂ as additives with synthetic biodegradable polymers has great potential by the synergistic effect in orthopedic applications.

Original language	English
Pages (from-to)	65-75
Number of pages	11
Journal	Materials Science and Engineering C
Volume	94
DOIs	https://doi.org/10.1016/j.msec.2018.09.011
Publication status	Published - 2019 Jan 1

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program ( 2017R1A2B3011121 ), Bio & Medical Technology Development Program ( 2014M3A9D3033887 and 2018M3A9E2024579 ) through National Research Foundation of Korea funded by the Ministry of Science and ICT (MSIT) and Core Materials Technology Development Program ( 10048019 ) funded by Ministry of Trade, Industry and Energy (MOTIE), Republic of Korea.

Publisher Copyright:
© 2018

Keywords

Anti-inflammation
Beta-tricalcium phosphate (β-TCP)
Magnesium hydroxide (Mg(OH))
Osteoconduction
Poly(lactic-co-glycolide) (PLGA)

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

Access to Document

10.1016/j.msec.2018.09.011

Cite this

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title = "Synergistically enhanced osteoconductivity and anti-inflammation of PLGA/β-TCP/Mg(OH)2 composite for orthopedic applications",

abstract = "Synthetic biodegradable polymers including poly(lactide-co-glycolide) (PLGA) have been widely used as alternatives to metallic implantable materials in the orthopedic field due to their superior biocompatibility and biodegradability. However, weak mechanical properties of the biodegradable polymers and inflammatory reaction caused by the acidic degradation products have limited their biomedical applications. In this study, we have developed a PLGA composite containing beta-tricalcium phosphate (β-TCP) and magnesium hydroxide (Mg(OH)2) as additives to improve mechanical, osteoconductivity, and anti-inflammation property of the biopolymer composite simultaneously. The β-TCP has an osteoconductive effect and the Mg(OH)2 has a pH neutralizing effect. The PLGA/inorganic composites were uniformly blended via a twin extrusion process. The mechanical property of the PLGA/β-TCP/Mg(OH)2 composite was improved compared to the pure PLGA. In particular, the addition of Mg(OH)2 suppressed the inflammatory reaction of normal human osteoblast (NHOst) cells and also inhibited the differentiation of pre-osteoclastic cells into osteoclasts. Moreover, synergistically upregulated late osteogenic differentiation of NHOst cells was observed on the PLGA/β-TCP/Mg(OH)2 composite. Taken all together, we believe that the use of β-TCP and Mg(OH)2 as additives with synthetic biodegradable polymers has great potential by the synergistic effect in orthopedic applications.",

keywords = "Anti-inflammation, Beta-tricalcium phosphate (β-TCP), Magnesium hydroxide (Mg(OH)), Osteoconduction, Poly(lactic-co-glycolide) (PLGA)",

author = "Lee, {Seul Ki} and Han, {Cheol Min} and Wooram Park and Kim, {Ik Hwan} and Joung, {Yoon Ki} and Han, {Dong Keun}",

note = "Funding Information: This work was supported by the Basic Science Research Program ( 2017R1A2B3011121 ), Bio & Medical Technology Development Program ( 2014M3A9D3033887 and 2018M3A9E2024579 ) through National Research Foundation of Korea funded by the Ministry of Science and ICT (MSIT) and Core Materials Technology Development Program ( 10048019 ) funded by Ministry of Trade, Industry and Energy (MOTIE), Republic of Korea. Publisher Copyright: {\textcopyright} 2018",

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T1 - Synergistically enhanced osteoconductivity and anti-inflammation of PLGA/β-TCP/Mg(OH)2 composite for orthopedic applications

AU - Lee, Seul Ki

AU - Han, Cheol Min

AU - Park, Wooram

AU - Kim, Ik Hwan

AU - Joung, Yoon Ki

AU - Han, Dong Keun

N1 - Funding Information: This work was supported by the Basic Science Research Program ( 2017R1A2B3011121 ), Bio & Medical Technology Development Program ( 2014M3A9D3033887 and 2018M3A9E2024579 ) through National Research Foundation of Korea funded by the Ministry of Science and ICT (MSIT) and Core Materials Technology Development Program ( 10048019 ) funded by Ministry of Trade, Industry and Energy (MOTIE), Republic of Korea. Publisher Copyright: © 2018

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Synthetic biodegradable polymers including poly(lactide-co-glycolide) (PLGA) have been widely used as alternatives to metallic implantable materials in the orthopedic field due to their superior biocompatibility and biodegradability. However, weak mechanical properties of the biodegradable polymers and inflammatory reaction caused by the acidic degradation products have limited their biomedical applications. In this study, we have developed a PLGA composite containing beta-tricalcium phosphate (β-TCP) and magnesium hydroxide (Mg(OH)2) as additives to improve mechanical, osteoconductivity, and anti-inflammation property of the biopolymer composite simultaneously. The β-TCP has an osteoconductive effect and the Mg(OH)2 has a pH neutralizing effect. The PLGA/inorganic composites were uniformly blended via a twin extrusion process. The mechanical property of the PLGA/β-TCP/Mg(OH)2 composite was improved compared to the pure PLGA. In particular, the addition of Mg(OH)2 suppressed the inflammatory reaction of normal human osteoblast (NHOst) cells and also inhibited the differentiation of pre-osteoclastic cells into osteoclasts. Moreover, synergistically upregulated late osteogenic differentiation of NHOst cells was observed on the PLGA/β-TCP/Mg(OH)2 composite. Taken all together, we believe that the use of β-TCP and Mg(OH)2 as additives with synthetic biodegradable polymers has great potential by the synergistic effect in orthopedic applications.

AB - Synthetic biodegradable polymers including poly(lactide-co-glycolide) (PLGA) have been widely used as alternatives to metallic implantable materials in the orthopedic field due to their superior biocompatibility and biodegradability. However, weak mechanical properties of the biodegradable polymers and inflammatory reaction caused by the acidic degradation products have limited their biomedical applications. In this study, we have developed a PLGA composite containing beta-tricalcium phosphate (β-TCP) and magnesium hydroxide (Mg(OH)2) as additives to improve mechanical, osteoconductivity, and anti-inflammation property of the biopolymer composite simultaneously. The β-TCP has an osteoconductive effect and the Mg(OH)2 has a pH neutralizing effect. The PLGA/inorganic composites were uniformly blended via a twin extrusion process. The mechanical property of the PLGA/β-TCP/Mg(OH)2 composite was improved compared to the pure PLGA. In particular, the addition of Mg(OH)2 suppressed the inflammatory reaction of normal human osteoblast (NHOst) cells and also inhibited the differentiation of pre-osteoclastic cells into osteoclasts. Moreover, synergistically upregulated late osteogenic differentiation of NHOst cells was observed on the PLGA/β-TCP/Mg(OH)2 composite. Taken all together, we believe that the use of β-TCP and Mg(OH)2 as additives with synthetic biodegradable polymers has great potential by the synergistic effect in orthopedic applications.

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KW - Beta-tricalcium phosphate (β-TCP)

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