Synovial fluid CD34 - CD44 + CD90 + mesenchymal stem cell levels are associated with the severity of primary knee osteoarthritis

D. H. Lee, C. H. Sonn, S. B. Han, Y. Oh, K. M. Lee, Soon-Hyuck Lee

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

To the best of our knowledge, no reports have directly compared synovial fluid (SF)- and synovial membrane (SM)-derived mesenchymal stem cells (MSCs) from primary knee osteoarthritis patients in terms of MSC proportion, either immediately after isolation or during culture. Any possible correlation between SM- and SF-MSC purity and osteoarthritis severity, also remains unclear. We therefore assessed quantitative and phenotypic differences in MSCs isolated from SF and SM. We also evaluated the correlation between sample MSC purity, and disease severity, in patients with osteoarthritis. The main result of the current study was thatthe mean SF-MSC proportion at passage 0 was negatively correlated with Kellgren-Lawrence (KL) grade (r=-0.565,. P=0.002). In addition, KL grade was a only significant independent negative predictor of SF-MSC proportion at passage 0 (β=-0.356,. P=0.039). Conclusively, the proportion of SF-MSCs in fresh samples, evaluated at the single cell level, was inversely correlated with osteoarthritis severity.

Original languageEnglish
Pages (from-to)106-109
Number of pages4
JournalOsteoarthritis and Cartilage
Volume20
Issue number2
DOIs
Publication statusPublished - 2012 Feb

Bibliographical note

Funding Information:
Research of this case was performed at Korea University Anam Hospital. This work (2011-0011149) was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology . C.H. Sonn and K.M. Lee were supported by a grant from KICOS (K20702001994 -11A0500-03610), the Converging Research Center Program through the Ministry of Education, Science and Technology (2011K000815), the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No.K20903001812-11E0100-01700) and Seoul R&BD Program No.10920. The authors thank Jung-Ik Lee (Department of Biomedical Science & Technology, Institute of Biomedical Science & Technology, Konkuk University) for help with the chondrogenesis culture.

Keywords

  • Mesenchymal stem cell
  • Osteoarthritis
  • Synovial fluid
  • Synovium

ASJC Scopus subject areas

  • Rheumatology
  • Biomedical Engineering
  • Orthopedics and Sports Medicine

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