Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles

Myung Ik Yoo; Yeong Ju Seo; Kyu Sil Choi; Jeong Sook Ha; Kyoungja Woo

doi:10.1016/j.colsurfb.2012.04.009

Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles

Myung Ik Yoo, Yeong Ju Seo, Kyu Sil Choi, Jeong Sook Ha, Kyoungja Woo

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin α _vβ ₃) and MCF-7 cells (human breast cancer cells, express low level of integrin α _vβ ₃) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs (SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin α _vβ ₃-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs.

Original language	English
Pages (from-to)	175-181
Number of pages	7
Journal	Colloids and Surfaces B: Biointerfaces
Volume	97
DOIs	https://doi.org/10.1016/j.colsurfb.2012.04.009
Publication status	Published - 2012 Sept 1

Bibliographical note

Funding Information:
This research was supported by Future Key Technology Program of Korea Institute of Science and Technology and by Future-based Technology Development Program (Green Nano Technology Development Program) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant number 2011-0030204).

Keywords

Cellular uptake
Lipophilic
Scatteredly cRGDfK-conjugated
Superparamagnetic iron oxide nanoparticles (SPIONs)
Ultrasmall
Water-dispersible

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.colsurfb.2012.04.009

Cite this

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title = "Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles",

abstract = "We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin α vβ 3) and MCF-7 cells (human breast cancer cells, express low level of integrin α vβ 3) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs (SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin α vβ 3-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs.",

keywords = "Cellular uptake, Lipophilic, Scatteredly cRGDfK-conjugated, Superparamagnetic iron oxide nanoparticles (SPIONs), Ultrasmall, Water-dispersible",

author = "Yoo, {Myung Ik} and Seo, {Yeong Ju} and Choi, {Kyu Sil} and Ha, {Jeong Sook} and Kyoungja Woo",

note = "Funding Information: This research was supported by Future Key Technology Program of Korea Institute of Science and Technology and by Future-based Technology Development Program (Green Nano Technology Development Program) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant number 2011-0030204). ",

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AU - Seo, Yeong Ju

AU - Choi, Kyu Sil

AU - Ha, Jeong Sook

AU - Woo, Kyoungja

N1 - Funding Information: This research was supported by Future Key Technology Program of Korea Institute of Science and Technology and by Future-based Technology Development Program (Green Nano Technology Development Program) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant number 2011-0030204).

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N2 - We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin α vβ 3) and MCF-7 cells (human breast cancer cells, express low level of integrin α vβ 3) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs (SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin α vβ 3-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs.

AB - We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin α vβ 3) and MCF-7 cells (human breast cancer cells, express low level of integrin α vβ 3) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs (SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin α vβ 3-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs.

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KW - Superparamagnetic iron oxide nanoparticles (SPIONs)

KW - Ultrasmall

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JO - Colloids and Surfaces B: Biointerfaces

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ER -

Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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