Abstract
We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin α vβ 3) and MCF-7 cells (human breast cancer cells, express low level of integrin α vβ 3) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs (SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin α vβ 3-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs.
Original language | English |
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Pages (from-to) | 175-181 |
Number of pages | 7 |
Journal | Colloids and Surfaces B: Biointerfaces |
Volume | 97 |
DOIs | |
Publication status | Published - 2012 Sept 1 |
Bibliographical note
Funding Information:This research was supported by Future Key Technology Program of Korea Institute of Science and Technology and by Future-based Technology Development Program (Green Nano Technology Development Program) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant number 2011-0030204).
Keywords
- Cellular uptake
- Lipophilic
- Scatteredly cRGDfK-conjugated
- Superparamagnetic iron oxide nanoparticles (SPIONs)
- Ultrasmall
- Water-dispersible
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry