Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles

Myung Ik Yoo, Yeong Ju Seo, Kyu Sil Choi, Jeong Sook Ha, Kyoungja Woo

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin α vβ 3) and MCF-7 cells (human breast cancer cells, express low level of integrin α vβ 3) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs (SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin α vβ 3-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Publication statusPublished - 2012 Sept 1


  • Cellular uptake
  • Lipophilic
  • Scatteredly cRGDfK-conjugated
  • Superparamagnetic iron oxide nanoparticles (SPIONs)
  • Ultrasmall
  • Water-dispersible

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry


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