Synthesis and cellular uptake of scatteredly cyclic RGDfK-conjugated superparamagnetic iron oxide nanoparticles

Myung Ik Yoo, Yeong Ju Seo, Kyu Sil Choi, Jeong Sook Ha, Kyoungja Woo

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    We prepared scatteredly cyclic RGDfK-conjugated water-dispersible superparamagnetic iron oxide nanoparticles (cRGDfK-WSPIONs) and investigated their cellular uptake to MS-1 cells (mouse endothelial cell lines, express integrin α vβ 3) and MCF-7 cells (human breast cancer cells, express low level of integrin α vβ 3) using in vitro MRI. The cRGDfK-WSPIONs were prepared from oleate-protected SPIONs (SPION-OA) as follows. Some oleates (OAs) on the SPION-OA were substituted by mercaptohexadecanoic acids (MHA) and cRGDfKs were conjugated to MHAs. Finally, the remaining OAs were substituted by mercaptopropionic acids without detaching preexisting cRGDfK-conjugated MHA ligands from the SPION surface. The cRGDfK-WSPIONs showed drastically higher cellular uptake than its corresponding control WSPIONs to MS-1 cells and also, showed higher selectivity to MS-1 cells than to MCF-7 cells, both implicating integrin α vβ 3-mediated cellular uptake of scatteredly cRGDfK-conjugated WSPIONs.

    Original languageEnglish
    Pages (from-to)175-181
    Number of pages7
    JournalColloids and Surfaces B: Biointerfaces
    Volume97
    DOIs
    Publication statusPublished - 2012 Sept 1

    Bibliographical note

    Funding Information:
    This research was supported by Future Key Technology Program of Korea Institute of Science and Technology and by Future-based Technology Development Program (Green Nano Technology Development Program) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant number 2011-0030204).

    Keywords

    • Cellular uptake
    • Lipophilic
    • Scatteredly cRGDfK-conjugated
    • Superparamagnetic iron oxide nanoparticles (SPIONs)
    • Ultrasmall
    • Water-dispersible

    ASJC Scopus subject areas

    • Biotechnology
    • Surfaces and Interfaces
    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry

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