Synthesis and evaluation of bone morphogenetic protein (BMP)-loaded hydroxyapatite microspheres for enhanced bone regeneration

Jaeuk Baek, Hyun Do Jung, Tae Sik Jang, Sung Won Kim, Min Ho Kang, Hyoun Ee Kim, Young Hag Koh

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


This study demonstrates a novel, simple way of synthesizing bone morphogenetic protein (BMP)-loaded hydroxyapatite (HA) microspheres for enhanced bone regeneration. To accomplish this, calcium phosphate cement (CPC) microspheres, consisting of α-TCP and TTCP powders, were prepared using water-in-oil (W/O) emulsion, followed by treatment at 37 °C for 3 days to convert the CPC to HA. The BMP-loaded HA microspheres with a well-defined spherical shape had a highly porous structure, which was created by the entanglement of precipitated HA crystals with a needle-like morphology. The unique porous structure with the bone mineral-like HA phase resulted in a considerable improvement in in vitro biocompatibility. In addition, the bone regeneration ability of the HA microspheres was significantly enhanced by BMP loading, which was examined by in vivo animal testing using a rabbit vertical guided bone regeneration model.

Original languageEnglish
Pages (from-to)7748-7756
Number of pages9
JournalCeramics International
Issue number6
Publication statusPublished - 2016 May 1

Bibliographical note

Funding Information:
This work was supported by the Industrial Strategic Technology Development Program (Contract no. 10045329 , Development of customized implant with porous structure for bone replacement) funded by the Ministry of Trade, Industry and Energy (MOTIE) , Republic of Korea and the Grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (Contract grant number: HI11C0388 ).

Publisher Copyright:
© 2016 Elsevier Ltd and Techna Group S.r.l.


  • B. Porosity
  • D. Apatite
  • E. Biomedical applications
  • Microsphere

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Process Chemistry and Technology
  • Surfaces, Coatings and Films
  • Materials Chemistry


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