Abstract
A green, simple, and effective approach was performed to synthesize potent silver nanoparticles (SNPs) using bacterial exopolysaccharide as both a reducing and stabilizing agent. The synthesized SNPs were characterized using UV-vis spectroscopy, transmission electron microscopy, energy dispersive X-ray analysis, X-ray diffraction, and Fourier-transform-infrared spectra analyses. The SNPs varied in shape and were multidispersed with a mean diameter of 10 nm ranging from 2 to 15 nm and were stable up to 2 months at room temperature. The antimicrobial activity of the SNPs was analyzed against bacterial and fungal pathogens using the agar well diffusion method. Dose dependent inhibition was observed for all bacterial pathogens. The multidrug resistant pathogens P. aeruginosa and K. pneumonia were found to be more susceptible to the SNPs than the food borne pathogen L. monocytogenes. The fungi Aspergillus spp. exhibited a maximum zone of inhibition compared to that of Penicillum spp. These results suggest that exopolysaccharide-stabilized SNPs can be used as an antimicrobial agent for various biomedical applications.
Original language | English |
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Pages (from-to) | 1099-1106 |
Number of pages | 8 |
Journal | Process Biochemistry |
Volume | 48 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2013 Jul |
Bibliographical note
Copyright:Copyright 2013 Elsevier B.V., All rights reserved.
Keywords
- Antimicrobial activity
- Exopolysaccharide
- Lactobacillus rhamnosus
- Silver nanoparticles
- TEM and XRD
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology