Synthesis of au–cu alloy nanoparticles as peroxidase mimetics for h2o2 and glucose colorimetric detection

Cun Liu, Sang Hyuk Im, Taekyung Yu

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


The detection of hydrogen peroxide (H2O2 ) is essential in many research fields, including medical diagnosis, food safety, and environmental monitoring. In this context, Au-based bimetallic alloy nanomaterials have attracted increasing attention as an alternative to enzymes due to their superior catalytic activity. In this study, we report a coreduction synthesis of gold–copper (Au–Cu) alloy nanoparticles in aqueous phase. By controlling the amount of Au and Cu precursors, the Au/Cu molar ratio of the nanoparticles can be tuned from 1/0.1 to 1/2. The synthesized Au–Cu alloy nanoparticles show good peroxidase-like catalytic activity and high selectivity for the H2O2-mediated oxidation of 3,3,5,5-tetramethylbenzidine (TMB, colorless) to TMB oxide (blue). The Au–Cu nanoparticles with an Au/Cu molar ratio of 1/2 exhibit high catalytic activity in the H2O2 colorimetric detection, with a limit of detection of 0.141 µM in the linear range of 1–10 µM and a correlation coefficient R2 = 0.991. Furthermore, the Au–Cu alloy nanoparticles can also efficiently detect glucose in the presence of glucose oxidase (GOx), and the detection limit is as low as 0.26 µM.

Original languageEnglish
Article number343
Pages (from-to)1-12
Number of pages12
Issue number3
Publication statusPublished - 2021 Mar

Bibliographical note

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.


  • Au–Cu alloy nanoparticles
  • HO colorimetric detection
  • Peroxidase mimetic

ASJC Scopus subject areas

  • Catalysis
  • Physical and Theoretical Chemistry


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