Synthesis of branched oligosaccharides from starch by two amylases cloned from bacillus licheniformis

In Cheol Kim; Sang Ho Yoo; Sung Joon Lee; Byung Ha Oh; Jung Wan Kim; Kwan Hwa Park

doi:10.1080/bbb.58.416

Synthesis of branched oligosaccharides from starch by two amylases cloned from bacillus licheniformis

In Cheol Kim, Sang Ho Yoo, Sung Joon Lee, Byung Ha Oh, Jung Wan Kim, Kwan Hwa Park

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

An E. coli cell extract containing thermostable α-amylase and maltogenic amylase expressed from the clone pTMA322 was used to produce an anomalously linked oligosaccharides (Alo) mixture from starch. The liquefaction and saccharification of starch were done by one-step procedure using the above cell extract. The resulted Alo mixture contained over 40% oligosaccharides having DP 3 or more including branched forms.

Original language	English
Pages (from-to)	416-418
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	58
Issue number	2
DOIs	https://doi.org/10.1080/bbb.58.416
Publication status	Published - 1994 Jan
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments. Dr. J. W. Kim is supported by the Korea Research Foundation. This work was supported by Research Center for New Bio-Materials in Agriculture.

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Synthesis of branched oligosaccharides from starch by two amylases cloned from bacillus licheniformis",

abstract = "An E. coli cell extract containing thermostable α-amylase and maltogenic amylase expressed from the clone pTMA322 was used to produce an anomalously linked oligosaccharides (Alo) mixture from starch. The liquefaction and saccharification of starch were done by one-step procedure using the above cell extract. The resulted Alo mixture contained over 40% oligosaccharides having DP 3 or more including branched forms.",

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note = "Funding Information: Acknowledgments. Dr. J. W. Kim is supported by the Korea Research Foundation. This work was supported by Research Center for New Bio-Materials in Agriculture.",

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AU - Yoo, Sang Ho

AU - Lee, Sung Joon

AU - Oh, Byung Ha

AU - Kim, Jung Wan

AU - Park, Kwan Hwa

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AB - An E. coli cell extract containing thermostable α-amylase and maltogenic amylase expressed from the clone pTMA322 was used to produce an anomalously linked oligosaccharides (Alo) mixture from starch. The liquefaction and saccharification of starch were done by one-step procedure using the above cell extract. The resulted Alo mixture contained over 40% oligosaccharides having DP 3 or more including branched forms.

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ER -

Synthesis of branched oligosaccharides from starch by two amylases cloned from bacillus licheniformis

Abstract

Bibliographical note

ASJC Scopus subject areas

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