Synthesis of streptavidin-FITC-conjugated core-shell Fe3O4-Au nanocrystals and their application for the purification of CD4+ lymphocytes

Hong Ling Liu; Chung Hee Sonn; Jun Hua Wu; Kyung Mi Lee; Young Keun Kim

doi:10.1016/j.biomaterials.2008.06.031

Synthesis of streptavidin-FITC-conjugated core-shell Fe₃O₄-Au nanocrystals and their application for the purification of CD4⁺ lymphocytes

Hong Ling Liu, Chung Hee Sonn, Jun Hua Wu, Kyung Mi Lee, Young Keun Kim

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

Abstract

We explored the feasibility of magnetite (Fe₃O₄)-gold (Au) core-shell nanocrystals as a useful vehicle for biomedical application such as cell separation. Streptavidin-fluorescein isothiocyanate (STA-FITC) was conjugated to the surface of the Fe₃O₄-Au core-shell nanocrystals using a carbodimide activation protocol. These nanocrystals were further tested for their ability to bind CD4⁺ T lymphocytes, bound to biotin-labeled anti-CD4 mAbs, isolated from the spleen of C57BL/6 mice. Our data show that the Fe₃O₄-Au nanocrystals successfully pulled down CD4⁺ T lymphocytes from the whole splenocytes with high specificity. Therefore, our nanocrystals provide an efficient tool for the cell separation process and further present the dramatic potential to be applied to other areas of biomedical application including diagnosis, monitoring, and treatment of human diseases.

Original language	English
Pages (from-to)	4003-4011
Number of pages	9
Journal	Biomaterials
Volume	29
Issue number	29
DOIs	https://doi.org/10.1016/j.biomaterials.2008.06.031
Publication status	Published - 2008 Oct

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Engineering Foundation through the Pioneer Program funded by the Ministry of Education, Science and Technology, the Korea Health 21 R&D Project grant A050750, and the Seoul R&BD Program No. 10920. K. M. Lee is supported by the Korea Foundation for International Cooperation of Science and Technology grants K20704000007-07A0500-00710 and K20601000002-07E0100-00240, and the Innovative Research Institute for Cell Therapy grant A062260. C. H. Sonn is supported by the National Nuclear R&D program grant BAERI.

Keywords

Core-shell nanocrystals
Fe3O4-Au
Fluorescence-activated cell sorting
Magnetic activated cell separation
Streptavidin

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biomaterials.2008.06.031

Cite this

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title = "Synthesis of streptavidin-FITC-conjugated core-shell Fe3O4-Au nanocrystals and their application for the purification of CD4+ lymphocytes",

abstract = "We explored the feasibility of magnetite (Fe3O4)-gold (Au) core-shell nanocrystals as a useful vehicle for biomedical application such as cell separation. Streptavidin-fluorescein isothiocyanate (STA-FITC) was conjugated to the surface of the Fe3O4-Au core-shell nanocrystals using a carbodimide activation protocol. These nanocrystals were further tested for their ability to bind CD4+ T lymphocytes, bound to biotin-labeled anti-CD4 mAbs, isolated from the spleen of C57BL/6 mice. Our data show that the Fe3O4-Au nanocrystals successfully pulled down CD4+ T lymphocytes from the whole splenocytes with high specificity. Therefore, our nanocrystals provide an efficient tool for the cell separation process and further present the dramatic potential to be applied to other areas of biomedical application including diagnosis, monitoring, and treatment of human diseases.",

keywords = "Core-shell nanocrystals, Fe3O4-Au, Fluorescence-activated cell sorting, Magnetic activated cell separation, Streptavidin",

author = "Liu, {Hong Ling} and Sonn, {Chung Hee} and Wu, {Jun Hua} and Lee, {Kyung Mi} and Kim, {Young Keun}",

note = "Funding Information: This work was supported by the Korea Science and Engineering Foundation through the Pioneer Program funded by the Ministry of Education, Science and Technology, the Korea Health 21 R&D Project grant A050750, and the Seoul R&BD Program No. 10920. K. M. Lee is supported by the Korea Foundation for International Cooperation of Science and Technology grants K20704000007-07A0500-00710 and K20601000002-07E0100-00240, and the Innovative Research Institute for Cell Therapy grant A062260. C. H. Sonn is supported by the National Nuclear R&D program grant BAERI.",

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N1 - Funding Information: This work was supported by the Korea Science and Engineering Foundation through the Pioneer Program funded by the Ministry of Education, Science and Technology, the Korea Health 21 R&D Project grant A050750, and the Seoul R&BD Program No. 10920. K. M. Lee is supported by the Korea Foundation for International Cooperation of Science and Technology grants K20704000007-07A0500-00710 and K20601000002-07E0100-00240, and the Innovative Research Institute for Cell Therapy grant A062260. C. H. Sonn is supported by the National Nuclear R&D program grant BAERI.

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N2 - We explored the feasibility of magnetite (Fe3O4)-gold (Au) core-shell nanocrystals as a useful vehicle for biomedical application such as cell separation. Streptavidin-fluorescein isothiocyanate (STA-FITC) was conjugated to the surface of the Fe3O4-Au core-shell nanocrystals using a carbodimide activation protocol. These nanocrystals were further tested for their ability to bind CD4+ T lymphocytes, bound to biotin-labeled anti-CD4 mAbs, isolated from the spleen of C57BL/6 mice. Our data show that the Fe3O4-Au nanocrystals successfully pulled down CD4+ T lymphocytes from the whole splenocytes with high specificity. Therefore, our nanocrystals provide an efficient tool for the cell separation process and further present the dramatic potential to be applied to other areas of biomedical application including diagnosis, monitoring, and treatment of human diseases.

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