Synthesis of trans-10,cis-12 conjugated linoleic acid-enriched triacylglycerols via two-step lipase-catalyzed esterification

Ingu Kang, Hyo Jeong Bang, In Hwan Kim, Hee Don Choi, Byung Hee Kim

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


This study aimed to synthesize trans-10,. cis-12 conjugated linoleic acid (t10,. c12-CLA)-enriched triacylglycerols (TAGs) with potential anti-obesity effects via a two-step lipase-catalyzed reaction. Commercial CLA isomer mixtures, containing 33.3g/100g t10,. c12-CLA, were esterified with dodecan-1-ol to selectively enrich the t10,. c12-CLA in a free fatty acid (FFA) fraction. The reaction was performed in a recirculating packed bed reactor using Candida rugosa lipase (immobilized on Immobead 150) as the biocatalyst. An FFA fraction containing 54.7g/100g t10,. c12-CLA was produced in a yield of 21.8g/100g initial t10,. c12-CLA under the optimal conditions, i.e., temperature, 20°C; CLA mixtures-to-dodecan-1-ol molar ratio, 1:1; water content, zero (no added water); reaction time, 36h. A t10,. c12-CLA-enriched FFA fraction was esterified with glycerol to prepare t10,. c12-CLA-enriched TAGs. The reaction was performed in a stirred batch reactor using Candida antarctica lipase B (immobilized on macroporous acrylic resin) as the biocatalyst. The optimal combination of temperature, glycerol-to-FFA fraction molar ratio, enzyme loading, reaction time, and vacuum level was 60°C, 1:3, 10g/100g (based on total substrates), 12h, and 0.4kPa, respectively. Under these conditions, the TAG content reached 93.7g/100g and t10,. c12-CLA was evenly distributed throughout the glycerol backbone of the TAG.

Original languageEnglish
Pages (from-to)249-256
Number of pages8
Issue number1
Publication statusPublished - 2015 Jun 1


  • Esterification
  • Lipase
  • Recirculating packed bed reactor
  • Trans-10,cis-12 conjugated linoleic acid
  • Triacylglycerol

ASJC Scopus subject areas

  • Food Science


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