TY - JOUR
T1 - Synthesis of trans-10,cis-12 conjugated linoleic acid-enriched triacylglycerols via two-step lipase-catalyzed esterification
AU - Kang, Ingu
AU - Bang, Hyo Jeong
AU - Kim, In Hwan
AU - Choi, Hee Don
AU - Kim, Byung Hee
N1 - Funding Information:
This study was supported by a research grant from the Korea Food Research Institute .
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - This study aimed to synthesize trans-10,. cis-12 conjugated linoleic acid (t10,. c12-CLA)-enriched triacylglycerols (TAGs) with potential anti-obesity effects via a two-step lipase-catalyzed reaction. Commercial CLA isomer mixtures, containing 33.3g/100g t10,. c12-CLA, were esterified with dodecan-1-ol to selectively enrich the t10,. c12-CLA in a free fatty acid (FFA) fraction. The reaction was performed in a recirculating packed bed reactor using Candida rugosa lipase (immobilized on Immobead 150) as the biocatalyst. An FFA fraction containing 54.7g/100g t10,. c12-CLA was produced in a yield of 21.8g/100g initial t10,. c12-CLA under the optimal conditions, i.e., temperature, 20°C; CLA mixtures-to-dodecan-1-ol molar ratio, 1:1; water content, zero (no added water); reaction time, 36h. A t10,. c12-CLA-enriched FFA fraction was esterified with glycerol to prepare t10,. c12-CLA-enriched TAGs. The reaction was performed in a stirred batch reactor using Candida antarctica lipase B (immobilized on macroporous acrylic resin) as the biocatalyst. The optimal combination of temperature, glycerol-to-FFA fraction molar ratio, enzyme loading, reaction time, and vacuum level was 60°C, 1:3, 10g/100g (based on total substrates), 12h, and 0.4kPa, respectively. Under these conditions, the TAG content reached 93.7g/100g and t10,. c12-CLA was evenly distributed throughout the glycerol backbone of the TAG.
AB - This study aimed to synthesize trans-10,. cis-12 conjugated linoleic acid (t10,. c12-CLA)-enriched triacylglycerols (TAGs) with potential anti-obesity effects via a two-step lipase-catalyzed reaction. Commercial CLA isomer mixtures, containing 33.3g/100g t10,. c12-CLA, were esterified with dodecan-1-ol to selectively enrich the t10,. c12-CLA in a free fatty acid (FFA) fraction. The reaction was performed in a recirculating packed bed reactor using Candida rugosa lipase (immobilized on Immobead 150) as the biocatalyst. An FFA fraction containing 54.7g/100g t10,. c12-CLA was produced in a yield of 21.8g/100g initial t10,. c12-CLA under the optimal conditions, i.e., temperature, 20°C; CLA mixtures-to-dodecan-1-ol molar ratio, 1:1; water content, zero (no added water); reaction time, 36h. A t10,. c12-CLA-enriched FFA fraction was esterified with glycerol to prepare t10,. c12-CLA-enriched TAGs. The reaction was performed in a stirred batch reactor using Candida antarctica lipase B (immobilized on macroporous acrylic resin) as the biocatalyst. The optimal combination of temperature, glycerol-to-FFA fraction molar ratio, enzyme loading, reaction time, and vacuum level was 60°C, 1:3, 10g/100g (based on total substrates), 12h, and 0.4kPa, respectively. Under these conditions, the TAG content reached 93.7g/100g and t10,. c12-CLA was evenly distributed throughout the glycerol backbone of the TAG.
KW - Esterification
KW - Lipase
KW - Recirculating packed bed reactor
KW - Trans-10,cis-12 conjugated linoleic acid
KW - Triacylglycerol
UR - http://www.scopus.com/inward/record.url?scp=84923222082&partnerID=8YFLogxK
U2 - 10.1016/j.lwt.2015.01.041
DO - 10.1016/j.lwt.2015.01.041
M3 - Article
AN - SCOPUS:84923222082
SN - 0023-6438
VL - 62
SP - 249
EP - 256
JO - LWT - Food Science and Technology
JF - LWT - Food Science and Technology
IS - 1
ER -
