Syringaresinol induces mitochondrial biogenesis through activation of PPARβ pathway in skeletal muscle cells

Trung Thanh Thach, Chan Kyu Lee, Hyun woo Park, Sang Jun Lee, Sung Joon Lee

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Activation of peroxisome proliferator-activated receptors (PPARs) plays a crucial role in cellular energy metabolism that directly impacts mitochondrial biogenesis. In this study, we demonstrate that syringaresinol, a pharmacological lignan extracted from Panax ginseng berry, moderately binds to and activates PPARβ with KDand EC50values of 27.62 ± 15.76 μM and 18.11 ± 4.77 μM, respectively. Subsequently, the expression of peroxisome proliferator-activated receptor γ coactivator-1α together with PPARβ transcriptional targets, mitochondrial carnitine palmitoyltransferase 1 and uncoupling protein 2, was also enhanced in terms of both mRNA and protein levels. The activation of these proteins induced mitochondrial biogenesis by enrichment of mitochondrial replication and density within C2C12 myotubes. Importantly, knockdown of PPARβ reduced the syringaresinol-induced protein expression followed by the significant reduction of mitochondrial biogenesis. Taken together, our results indicate that syringaresinol induces mitochondrial biogenesis by activating PPARβ pathway.

Original languageEnglish
Pages (from-to)3978-3983
Number of pages6
JournalBioorganic and Medicinal Chemistry Letters
Issue number16
Publication statusPublished - 2016

Bibliographical note

Funding Information:
This work was carried out with the support of the ‘Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01052801 and PJ010090032015)’, Rural Development Administration (RDA), Republic of Korea, and was supported by a grant (14162MFDS973) from the Korean Ministry of Food and Drug Safety, and Korea University Grant, Republic of Korea.

Publisher Copyright:
© 2016 Elsevier Ltd


  • Mitochondrial biogenesis
  • PPARβ
  • Skeletal muscle
  • Syringaresinol

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry


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