Tailoring the supramolecular structure of aminated polyrotaxanes toward enhanced cellular internalization

Nanako Yokoyama; Ji Hun Seo; Atsushi Tamura; Yoshihiro Sasaki; Nobuhiko Yui

doi:10.1002/mabi.201300198

Tailoring the supramolecular structure of aminated polyrotaxanes toward enhanced cellular internalization

Nanako Yokoyama, Ji Hun Seo, Atsushi Tamura, Yoshihiro Sasaki, Nobuhiko Yui

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The effects of the supramolecular polyrotaxane (PRX) structure on cellular internalization are investigated by flow cytometry and confocal laser scanning microscopy. AF-545-labeled aminated PRXs (APRXs) containing different numbers of threaded α-cyclodextrins (CDs) and amino groups are synthesized; their cellular uptakes are analyzed using HeLa cells in serum. The APRX threaded CD number is discovered to be a more critical factor for enhancing cellular internalization than the APRX amine content. Additionally, APRXs are demonstrated to be more easily internalized than conventional linear cationic macromolecules. Because increased numbers of threaded CDs are related to increased PRX rigidity, the PRX rigid frame resulting from CD molecules threaded on a poly(ethylene glycol) (PEG) chain is suitable for intracellular tools in therapy and diagnosis. The effects of the supramolecular polyrotaxane (PRX) structure on cellular internalization are investigated. The PRX threaded α-cyclodextrin number is discovered to be a more critical factor for enhancing cellular internalization than the PRX amine content. Additionally, aminated PRXs are demonstrated to be more easily internalized than conventional linear cationic macromolecules.

Original language	English
Pages (from-to)	359-368
Number of pages	10
Journal	Macromolecular Bioscience
Volume	14
Issue number	3
DOIs	https://doi.org/10.1002/mabi.201300198
Publication status	Published - 2014 Mar
Externally published	Yes

Keywords

cellular internalization
cyclodextrin
electrostatic interaction
polyrotaxane
supramolecular structures

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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10.1002/mabi.201300198

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abstract = "The effects of the supramolecular polyrotaxane (PRX) structure on cellular internalization are investigated by flow cytometry and confocal laser scanning microscopy. AF-545-labeled aminated PRXs (APRXs) containing different numbers of threaded α-cyclodextrins (CDs) and amino groups are synthesized; their cellular uptakes are analyzed using HeLa cells in serum. The APRX threaded CD number is discovered to be a more critical factor for enhancing cellular internalization than the APRX amine content. Additionally, APRXs are demonstrated to be more easily internalized than conventional linear cationic macromolecules. Because increased numbers of threaded CDs are related to increased PRX rigidity, the PRX rigid frame resulting from CD molecules threaded on a poly(ethylene glycol) (PEG) chain is suitable for intracellular tools in therapy and diagnosis. The effects of the supramolecular polyrotaxane (PRX) structure on cellular internalization are investigated. The PRX threaded α-cyclodextrin number is discovered to be a more critical factor for enhancing cellular internalization than the PRX amine content. Additionally, aminated PRXs are demonstrated to be more easily internalized than conventional linear cationic macromolecules.",

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author = "Nanako Yokoyama and Seo, {Ji Hun} and Atsushi Tamura and Yoshihiro Sasaki and Nobuhiko Yui",

year = "2014",

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AU - Yokoyama, Nanako

AU - Seo, Ji Hun

AU - Tamura, Atsushi

AU - Sasaki, Yoshihiro

AU - Yui, Nobuhiko

PY - 2014/3

Y1 - 2014/3

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AB - The effects of the supramolecular polyrotaxane (PRX) structure on cellular internalization are investigated by flow cytometry and confocal laser scanning microscopy. AF-545-labeled aminated PRXs (APRXs) containing different numbers of threaded α-cyclodextrins (CDs) and amino groups are synthesized; their cellular uptakes are analyzed using HeLa cells in serum. The APRX threaded CD number is discovered to be a more critical factor for enhancing cellular internalization than the APRX amine content. Additionally, APRXs are demonstrated to be more easily internalized than conventional linear cationic macromolecules. Because increased numbers of threaded CDs are related to increased PRX rigidity, the PRX rigid frame resulting from CD molecules threaded on a poly(ethylene glycol) (PEG) chain is suitable for intracellular tools in therapy and diagnosis. The effects of the supramolecular polyrotaxane (PRX) structure on cellular internalization are investigated. The PRX threaded α-cyclodextrin number is discovered to be a more critical factor for enhancing cellular internalization than the PRX amine content. Additionally, aminated PRXs are demonstrated to be more easily internalized than conventional linear cationic macromolecules.

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Tailoring the supramolecular structure of aminated polyrotaxanes toward enhanced cellular internalization

Abstract

Keywords

ASJC Scopus subject areas

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