Targeted Mutagenesis in Mice Using a Base Editor

Tae Yeong Jeong, Soo Yeon Lim, Je Kyung Seong, Kyoungmi Kim

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Base editors, such as cytosine and adenine base editors, are composed of nickase Cas9 (nCas9) and deaminase and serve as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions. They are mainly the most effective genome editing tools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other nucleotide substitutions, such as C-to-G conversions. Here, we introduce a method for generating mouse models with point mutations using a base editing system.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages99-119
    Number of pages21
    DOIs
    Publication statusPublished - 2023

    Publication series

    NameMethods in Molecular Biology
    Volume2606
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

    Bibliographical note

    Publisher Copyright:
    © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

    Keywords

    • Adenine base editor
    • CRISPR system
    • Cytosine base editor
    • Mouse model generation
    • Nucleotide substitution
    • Targeted mutagenesis

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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