Targeted Mutagenesis in Mice Using a Base Editor

Tae Yeong Jeong; Soo Yeon Lim; Je Kyung Seong; Kyoungmi Kim

doi:10.1007/978-1-0716-2879-9_9

Targeted Mutagenesis in Mice Using a Base Editor

Tae Yeong Jeong, Soo Yeon Lim, Je Kyung Seong, Kyoungmi Kim

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Base editors, such as cytosine and adenine base editors, are composed of nickase Cas9 (nCas9) and deaminase and serve as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions. They are mainly the most effective genome editing tools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other nucleotide substitutions, such as C-to-G conversions. Here, we introduce a method for generating mouse models with point mutations using a base editing system.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	99-119
Number of pages	21
DOIs	https://doi.org/10.1007/978-1-0716-2879-9_9
Publication status	Published - 2023

Publication series

Name	Methods in Molecular Biology
Volume	2606
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Adenine base editor
CRISPR system
Cytosine base editor
Mouse model generation
Nucleotide substitution
Targeted mutagenesis

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-2879-9_9

Cite this

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Targeted Mutagenesis in Mice Using a Base Editor

Abstract

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Bibliographical note

Keywords

ASJC Scopus subject areas

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