Abstract
Base editors, such as cytosine and adenine base editors, are composed of nickase Cas9 (nCas9) and deaminase and serve as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions. They are mainly the most effective genome editing tools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other nucleotide substitutions, such as C-to-G conversions. Here, we introduce a method for generating mouse models with point mutations using a base editing system.
Original language | English |
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Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 99-119 |
Number of pages | 21 |
DOIs | |
Publication status | Published - 2023 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 2606 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Keywords
- Adenine base editor
- CRISPR system
- Cytosine base editor
- Mouse model generation
- Nucleotide substitution
- Targeted mutagenesis
ASJC Scopus subject areas
- Molecular Biology
- Genetics