Targeted Mutagenesis in Mice Using a Base Editor

Tae Yeong Jeong, Soo Yeon Lim, Je Kyung Seong, Kyoungmi Kim

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Base editors, such as cytosine and adenine base editors, are composed of nickase Cas9 (nCas9) and deaminase and serve as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions. They are mainly the most effective genome editing tools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other nucleotide substitutions, such as C-to-G conversions. Here, we introduce a method for generating mouse models with point mutations using a base editing system.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages99-119
Number of pages21
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2606
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Adenine base editor
  • CRISPR system
  • Cytosine base editor
  • Mouse model generation
  • Nucleotide substitution
  • Targeted mutagenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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