@inbook{9b02cf2965894797984ad04f55f9d65e,
title = "Targeted Mutagenesis in Mice Using a Base Editor",
abstract = "Base editors, such as cytosine and adenine base editors, are composed of nickase Cas9 (nCas9) and deaminase and serve as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions. They are mainly the most effective genome editing tools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other nucleotide substitutions, such as C-to-G conversions. Here, we introduce a method for generating mouse models with point mutations using a base editing system.",
keywords = "Adenine base editor, CRISPR system, Cytosine base editor, Mouse model generation, Nucleotide substitution, Targeted mutagenesis",
author = "Jeong, {Tae Yeong} and Lim, {Soo Yeon} and Seong, {Je Kyung} and Kyoungmi Kim",
note = "Funding Information: This work was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) of Korea (NRF-2020M3A9D5A01082439 and NRF-2019R1A2C2087198). The illustrations were generated using BioRender.com. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2879-9_9",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "99--119",
booktitle = "Methods in Molecular Biology",
}