Abstract
Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models.
Original language | English |
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Article number | 170 |
Journal | Genome Biology |
Volume | 22 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2021 Dec |
Keywords
- Adamts20
- Chromatin-modulating peptides
- Dwarf phenotype
- Germline transmission
- Igf2
- Mouse cells and embryos
- Prime editor
- Proximal dead sgRNA
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Cell Biology