Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers

Jin Won Park; Dong June Ahn

doi:10.1016/j.colsurfb.2007.09.020

Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers

Jin Won Park^*
, Dong June Ahn

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Mixed dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) monolayers have been deposited on mica using Langmuir-Blodgett technique, as a model system for biomembranes. Nanometer-scale surface physical properties were quantitatively characterized with the gradual temperature change using the atomic force microscope. At 25 °C, tapping mode imaging revealed the clear phase-separation in the form of microscopic DPPC domain embedded in a DOPC matrix and the obvious step height between the higher DPPC phase and the lower DOPC phase. Surface force measurement made at 25 °C in contact mode showed significant contrasts in deformation elasticity, adhesion, and jump-to-surface. These physical property differences were kept below 40 °C, while they almost disappeared over 40 °C. In addition, the reversibility of the properties for the temperature change was also found.

Original language	English
Pages (from-to)	157-161
Number of pages	5
Journal	Colloids and Surfaces B: Biointerfaces
Volume	62
Issue number	1
DOIs	https://doi.org/10.1016/j.colsurfb.2007.09.020
Publication status	Published - 2008 Mar 15

Bibliographical note

Funding Information:
This research was supported by Gachon Bio-Nano Research Institute, Kyungwon University. We thank Prof. I.S. Yoo at the Kyungwon University and Dr. G.S. Lee at the Korea University for valuable discussions.

Keywords

Adhesion
Atomic force microscopy
Deformation elasticity
Jump-to-surface
Langmuir-Blodgett technique
Phase-separation

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2007.09.020

Cite this

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title = "Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers",

abstract = "Mixed dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) monolayers have been deposited on mica using Langmuir-Blodgett technique, as a model system for biomembranes. Nanometer-scale surface physical properties were quantitatively characterized with the gradual temperature change using the atomic force microscope. At 25 °C, tapping mode imaging revealed the clear phase-separation in the form of microscopic DPPC domain embedded in a DOPC matrix and the obvious step height between the higher DPPC phase and the lower DOPC phase. Surface force measurement made at 25 °C in contact mode showed significant contrasts in deformation elasticity, adhesion, and jump-to-surface. These physical property differences were kept below 40 °C, while they almost disappeared over 40 °C. In addition, the reversibility of the properties for the temperature change was also found.",

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author = "Park, \{Jin Won\} and Ahn, \{Dong June\}",

note = "Funding Information: This research was supported by Gachon Bio-Nano Research Institute, Kyungwon University. We thank Prof. I.S. Yoo at the Kyungwon University and Dr. G.S. Lee at the Korea University for valuable discussions.",

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doi = "10.1016/j.colsurfb.2007.09.020",

language = "English",

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T1 - Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers

AU - Park, Jin Won

AU - Ahn, Dong June

N1 - Funding Information: This research was supported by Gachon Bio-Nano Research Institute, Kyungwon University. We thank Prof. I.S. Yoo at the Kyungwon University and Dr. G.S. Lee at the Korea University for valuable discussions.

PY - 2008/3/15

Y1 - 2008/3/15

N2 - Mixed dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) monolayers have been deposited on mica using Langmuir-Blodgett technique, as a model system for biomembranes. Nanometer-scale surface physical properties were quantitatively characterized with the gradual temperature change using the atomic force microscope. At 25 °C, tapping mode imaging revealed the clear phase-separation in the form of microscopic DPPC domain embedded in a DOPC matrix and the obvious step height between the higher DPPC phase and the lower DOPC phase. Surface force measurement made at 25 °C in contact mode showed significant contrasts in deformation elasticity, adhesion, and jump-to-surface. These physical property differences were kept below 40 °C, while they almost disappeared over 40 °C. In addition, the reversibility of the properties for the temperature change was also found.

AB - Mixed dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine (DOPC) monolayers have been deposited on mica using Langmuir-Blodgett technique, as a model system for biomembranes. Nanometer-scale surface physical properties were quantitatively characterized with the gradual temperature change using the atomic force microscope. At 25 °C, tapping mode imaging revealed the clear phase-separation in the form of microscopic DPPC domain embedded in a DOPC matrix and the obvious step height between the higher DPPC phase and the lower DOPC phase. Surface force measurement made at 25 °C in contact mode showed significant contrasts in deformation elasticity, adhesion, and jump-to-surface. These physical property differences were kept below 40 °C, while they almost disappeared over 40 °C. In addition, the reversibility of the properties for the temperature change was also found.

KW - Adhesion

KW - Atomic force microscopy

KW - Deformation elasticity

KW - Jump-to-surface

KW - Langmuir-Blodgett technique

KW - Phase-separation

UR - https://www.scopus.com/pages/publications/38849103087

U2 - 10.1016/j.colsurfb.2007.09.020

DO - 10.1016/j.colsurfb.2007.09.020

M3 - Article

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AN - SCOPUS:38849103087

SN - 0927-7765

VL - 62

SP - 157

EP - 161

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 1

ER -

Temperature effect on nanometer-scale physical properties of mixed phospholipid monolayers

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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