Temporal Control of WNT Activity Regulates Tooth Number in Fish

J. S. Shim; B. Kim; H. C. Park; Jae Jun Ryu

doi:10.1177/0022034518811322

Temporal Control of WNT Activity Regulates Tooth Number in Fish

J. S. Shim
, B. Kim
, H. C. Park
, Jae Jun Ryu^*

^*Corresponding author for this work

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Wnts determine cell polarity, cell proliferation, and cell differentiation during embryogenesis and play an essential role during tooth development initiation and morphogenesis. Wnt/β-catenin signaling has a time-dependent role in development because various signaling molecules that mutually interact are involved in the pathway, and tight regulation of the pathway is essential for normal development. Studies investigating how the Wnt/β-catenin signaling pathway controls the different stages of tooth development are rare. Specifically, the effects of Wnt/β-catenin signaling loss of function on different stages of tooth development are currently unknown. Here, we report the stage-dependent role of Wnt/β-catenin signaling in tooth development. In vivo loss and gain of function of Wnt/β-catenin signaling were implemented through the genetic overexpression of DKK1 with heat shock–inducible transgenic models and the pharmacologic inhibition of β-catenin destruction complex formation in zebrafish, respectively. We demonstrated that transient inhibition of Wnt/β-catenin signaling interrupted tooth development in a stage-dependent manner and conditional activation of Wnt/β-catenin signaling during 4V morphogenesis inhibited the development of 3V. These findings suggest that Wnt/β-catenin signaling plays an important role in the morphogenesis of teeth and the initiation of sequential tooth development in a stage-dependent manner.

Original language	English
Pages (from-to)	339-346
Number of pages	8
Journal	Journal of Dental Research
Volume	98
Issue number	3
DOIs	https://doi.org/10.1177/0022034518811322
Publication status	Published - 2019 Mar 1

Bibliographical note

Funding Information:
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea, funded by the Ministry of Education (2017R1D1A1B03035919). The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article. The authors thank to JF Rawls for generous gift of a Tg(NFκB:EGFP) zebrafish.

Publisher Copyright:
© International & American Associations for Dental Research 2018.

Keywords

Wnt signaling pathway
double tooth
gain-of-function mutation
loss-of-function mutation
morphogenesis
zebrafish

ASJC Scopus subject areas

General Dentistry

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10.1177/0022034518811322

Cite this

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title = "Temporal Control of WNT Activity Regulates Tooth Number in Fish",

abstract = "Wnts determine cell polarity, cell proliferation, and cell differentiation during embryogenesis and play an essential role during tooth development initiation and morphogenesis. Wnt/β-catenin signaling has a time-dependent role in development because various signaling molecules that mutually interact are involved in the pathway, and tight regulation of the pathway is essential for normal development. Studies investigating how the Wnt/β-catenin signaling pathway controls the different stages of tooth development are rare. Specifically, the effects of Wnt/β-catenin signaling loss of function on different stages of tooth development are currently unknown. Here, we report the stage-dependent role of Wnt/β-catenin signaling in tooth development. In vivo loss and gain of function of Wnt/β-catenin signaling were implemented through the genetic overexpression of DKK1 with heat shock–inducible transgenic models and the pharmacologic inhibition of β-catenin destruction complex formation in zebrafish, respectively. We demonstrated that transient inhibition of Wnt/β-catenin signaling interrupted tooth development in a stage-dependent manner and conditional activation of Wnt/β-catenin signaling during 4V morphogenesis inhibited the development of 3V. These findings suggest that Wnt/β-catenin signaling plays an important role in the morphogenesis of teeth and the initiation of sequential tooth development in a stage-dependent manner.",

keywords = "Wnt signaling pathway, double tooth, gain-of-function mutation, loss-of-function mutation, morphogenesis, zebrafish",

author = "Shim, \{J. S.\} and B. Kim and Park, \{H. C.\} and Ryu, \{Jae Jun\}",

note = "Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea, funded by the Ministry of Education (2017R1D1A1B03035919). The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article. The authors thank to JF Rawls for generous gift of a Tg(NFκB:EGFP) zebrafish. Publisher Copyright: {\textcopyright} International \& American Associations for Dental Research 2018.",

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doi = "10.1177/0022034518811322",

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AU - Shim, J. S.

AU - Kim, B.

AU - Park, H. C.

AU - Ryu, Jae Jun

N1 - Funding Information: This research was supported by the Basic Science Research Program through the National Research Foundation of Korea, funded by the Ministry of Education (2017R1D1A1B03035919). The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article. The authors thank to JF Rawls for generous gift of a Tg(NFκB:EGFP) zebrafish. Publisher Copyright: © International & American Associations for Dental Research 2018.

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Wnts determine cell polarity, cell proliferation, and cell differentiation during embryogenesis and play an essential role during tooth development initiation and morphogenesis. Wnt/β-catenin signaling has a time-dependent role in development because various signaling molecules that mutually interact are involved in the pathway, and tight regulation of the pathway is essential for normal development. Studies investigating how the Wnt/β-catenin signaling pathway controls the different stages of tooth development are rare. Specifically, the effects of Wnt/β-catenin signaling loss of function on different stages of tooth development are currently unknown. Here, we report the stage-dependent role of Wnt/β-catenin signaling in tooth development. In vivo loss and gain of function of Wnt/β-catenin signaling were implemented through the genetic overexpression of DKK1 with heat shock–inducible transgenic models and the pharmacologic inhibition of β-catenin destruction complex formation in zebrafish, respectively. We demonstrated that transient inhibition of Wnt/β-catenin signaling interrupted tooth development in a stage-dependent manner and conditional activation of Wnt/β-catenin signaling during 4V morphogenesis inhibited the development of 3V. These findings suggest that Wnt/β-catenin signaling plays an important role in the morphogenesis of teeth and the initiation of sequential tooth development in a stage-dependent manner.

AB - Wnts determine cell polarity, cell proliferation, and cell differentiation during embryogenesis and play an essential role during tooth development initiation and morphogenesis. Wnt/β-catenin signaling has a time-dependent role in development because various signaling molecules that mutually interact are involved in the pathway, and tight regulation of the pathway is essential for normal development. Studies investigating how the Wnt/β-catenin signaling pathway controls the different stages of tooth development are rare. Specifically, the effects of Wnt/β-catenin signaling loss of function on different stages of tooth development are currently unknown. Here, we report the stage-dependent role of Wnt/β-catenin signaling in tooth development. In vivo loss and gain of function of Wnt/β-catenin signaling were implemented through the genetic overexpression of DKK1 with heat shock–inducible transgenic models and the pharmacologic inhibition of β-catenin destruction complex formation in zebrafish, respectively. We demonstrated that transient inhibition of Wnt/β-catenin signaling interrupted tooth development in a stage-dependent manner and conditional activation of Wnt/β-catenin signaling during 4V morphogenesis inhibited the development of 3V. These findings suggest that Wnt/β-catenin signaling plays an important role in the morphogenesis of teeth and the initiation of sequential tooth development in a stage-dependent manner.

KW - Wnt signaling pathway

KW - double tooth

KW - gain-of-function mutation

KW - loss-of-function mutation

KW - morphogenesis

KW - zebrafish

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SN - 0022-0345

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JO - Journal of Dental Research

JF - Journal of Dental Research

IS - 3

ER -

Temporal Control of WNT Activity Regulates Tooth Number in Fish

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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