Tenacibaculum tangerinum sp. nov., isolated from a tidal flat sediment

An orange-coloured bacterium, designated as strain GRR-S3-23^T, was isolated from a tidal flat sediment collected from Garorim Bay, Chuncheongbuk-do, Republic of Korea. Cells of GRR-S3-23^T were aerobic, Gram-stain-negative, rod-shaped and motile. GRR-S3-23^T grew at 18–40 °C (optimum, 30 °C), pH 7.0–9.0 (optimum, pH 7.0) and with 2–4% NaCl (optimum, 2–3% w/v). Results of 16S rRNA gene sequence analysis indicated that GRR-S3-23^T was closely related to Tenacibaculum aiptasiae a4^T (97.6%), followed by Tenacibaculum aestuarii SMK-4^T (97.5%), Tenacibaculum mesophilum MBIC 1140^T (97.4%), Tenacibaculum singaporense TLL-A2^T (97.3%), Tenacibaculum crassostreae JO-1^T (97.2%),and Tenacibaculum sediminilitoris YKTF-3^T (97.1%). The average amino acid identity values between GRR-S3-23^T and the related strains were 86.8–72.8%, the average nucleotide identity values were 83.3–74.1%, and the digital DNA–DNA hybridization values were 27.0–19.6%. GRR-S3-23^T possessed menaquinone-6 (MK-6) as major respiratory quinone and had summed feature 3 (C_{16: 1} ω7c and/ or C_{16: 1} ω6c, 20.6%) and iso-C_{15: 1} G (10.8%) as major fatty acids (>10.0%). The polar lipid profiles of GRR-S3-23^T contained phosphatidylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid, three unidentified lipids, one unidentified glycolipid and four unidentified phospholipids. The DNA G+C content of GRR-S3-23^T was 33.7%. On the basis of the results of the polyphasic analysis involving phylogenetic, phylogenomic, physiological and chemotaxonomic analyses described in this study, GRR-S3-23^T is considered to represent a novel species within the genus Tenacibaculum, for which the name Tenacibaculum tangerinum is proposed. The type strain is GRR-S3-23^T (=KCTC 102029^T=KACC 23271^T=JCM 36353^T).