The 5' untranslated region of cucumber mosaic virus RNA4 confers highly competitive activity on heterologous luciferase mRNA in cell-free translation

Chang Seob Kwon, Kyung Hee Paek, Won Il Chung

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    The cell-free translation of virion RNAs of several tripartite RNA viruses has shown that RNA4, a subgenomic RNA, is more competitive than other virion RNAs. Recently, the 3' untranslated region (UTR) of alfalfa mosaic virus (AMV) RNA4 was identified to be a competitive determinant. In this study, we observed that the RNA4 of cucumber mosaic virus (CMV), another tripartite RNA virus, was also found to be a strong competitor in translational competition among CMV virion RNAs. To identify the competitive determinant of CMV RNA4, we constructed various chimeric luciferase mRNAs containing RNA4 and/or vector-derived UTRs. The relative translations of luciferase-containing mRNA in the presence of a competitor mRNA showed that the 5' UTR, not the 3' UTR, substantially contributed to the highly competitive activity of CMV RNA4.

    Original languageEnglish
    Pages (from-to)89-93
    Number of pages5
    JournalFEBS Letters
    Volume422
    Issue number1
    DOIs
    Publication statusPublished - 1998 Jan 23

    Bibliographical note

    Funding Information:
    This research was supported partly by Korea Science and Engineering Foundation and partly by the Genetic Engineering Research Fund of the Korea Ministry of Education.

    Keywords

    • 5' Untranslated region
    • Competitive determinant
    • Cucumber mosaic virus
    • Translation efficiency
    • mRNA competition

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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