The 5' untranslated region of cucumber mosaic virus RNA4 confers highly competitive activity on heterologous luciferase mRNA in cell-free translation

Chang Seob Kwon; Kyung Hee Paek; Won Il Chung

doi:10.1016/S0014-5793(97)01599-8

The 5' untranslated region of cucumber mosaic virus RNA4 confers highly competitive activity on heterologous luciferase mRNA in cell-free translation

Chang Seob Kwon, Kyung Hee Paek, Won Il Chung

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The cell-free translation of virion RNAs of several tripartite RNA viruses has shown that RNA4, a subgenomic RNA, is more competitive than other virion RNAs. Recently, the 3' untranslated region (UTR) of alfalfa mosaic virus (AMV) RNA4 was identified to be a competitive determinant. In this study, we observed that the RNA4 of cucumber mosaic virus (CMV), another tripartite RNA virus, was also found to be a strong competitor in translational competition among CMV virion RNAs. To identify the competitive determinant of CMV RNA4, we constructed various chimeric luciferase mRNAs containing RNA4 and/or vector-derived UTRs. The relative translations of luciferase-containing mRNA in the presence of a competitor mRNA showed that the 5' UTR, not the 3' UTR, substantially contributed to the highly competitive activity of CMV RNA4.

Original language	English
Pages (from-to)	89-93
Number of pages	5
Journal	FEBS Letters
Volume	422
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(97)01599-8
Publication status	Published - 1998 Jan 23

Bibliographical note

Funding Information:
This research was supported partly by Korea Science and Engineering Foundation and partly by the Genetic Engineering Research Fund of the Korea Ministry of Education.

Keywords

5' Untranslated region
Competitive determinant
Cucumber mosaic virus
Translation efficiency
mRNA competition

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(97)01599-8

Cite this

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title = "The 5' untranslated region of cucumber mosaic virus RNA4 confers highly competitive activity on heterologous luciferase mRNA in cell-free translation",

abstract = "The cell-free translation of virion RNAs of several tripartite RNA viruses has shown that RNA4, a subgenomic RNA, is more competitive than other virion RNAs. Recently, the 3' untranslated region (UTR) of alfalfa mosaic virus (AMV) RNA4 was identified to be a competitive determinant. In this study, we observed that the RNA4 of cucumber mosaic virus (CMV), another tripartite RNA virus, was also found to be a strong competitor in translational competition among CMV virion RNAs. To identify the competitive determinant of CMV RNA4, we constructed various chimeric luciferase mRNAs containing RNA4 and/or vector-derived UTRs. The relative translations of luciferase-containing mRNA in the presence of a competitor mRNA showed that the 5' UTR, not the 3' UTR, substantially contributed to the highly competitive activity of CMV RNA4.",

keywords = "5' Untranslated region, Competitive determinant, Cucumber mosaic virus, Translation efficiency, mRNA competition",

author = "Kwon, {Chang Seob} and Paek, {Kyung Hee} and Chung, {Won Il}",

note = "Funding Information: This research was supported partly by Korea Science and Engineering Foundation and partly by the Genetic Engineering Research Fund of the Korea Ministry of Education.",

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AU - Kwon, Chang Seob

AU - Paek, Kyung Hee

AU - Chung, Won Il

N1 - Funding Information: This research was supported partly by Korea Science and Engineering Foundation and partly by the Genetic Engineering Research Fund of the Korea Ministry of Education.

PY - 1998/1/23

Y1 - 1998/1/23

N2 - The cell-free translation of virion RNAs of several tripartite RNA viruses has shown that RNA4, a subgenomic RNA, is more competitive than other virion RNAs. Recently, the 3' untranslated region (UTR) of alfalfa mosaic virus (AMV) RNA4 was identified to be a competitive determinant. In this study, we observed that the RNA4 of cucumber mosaic virus (CMV), another tripartite RNA virus, was also found to be a strong competitor in translational competition among CMV virion RNAs. To identify the competitive determinant of CMV RNA4, we constructed various chimeric luciferase mRNAs containing RNA4 and/or vector-derived UTRs. The relative translations of luciferase-containing mRNA in the presence of a competitor mRNA showed that the 5' UTR, not the 3' UTR, substantially contributed to the highly competitive activity of CMV RNA4.

AB - The cell-free translation of virion RNAs of several tripartite RNA viruses has shown that RNA4, a subgenomic RNA, is more competitive than other virion RNAs. Recently, the 3' untranslated region (UTR) of alfalfa mosaic virus (AMV) RNA4 was identified to be a competitive determinant. In this study, we observed that the RNA4 of cucumber mosaic virus (CMV), another tripartite RNA virus, was also found to be a strong competitor in translational competition among CMV virion RNAs. To identify the competitive determinant of CMV RNA4, we constructed various chimeric luciferase mRNAs containing RNA4 and/or vector-derived UTRs. The relative translations of luciferase-containing mRNA in the presence of a competitor mRNA showed that the 5' UTR, not the 3' UTR, substantially contributed to the highly competitive activity of CMV RNA4.

KW - 5' Untranslated region

KW - Competitive determinant

KW - Cucumber mosaic virus

KW - Translation efficiency

KW - mRNA competition

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IS - 1

ER -

The 5' untranslated region of cucumber mosaic virus RNA4 confers highly competitive activity on heterologous luciferase mRNA in cell-free translation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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