TY - JOUR
T1 - The 5' untranslated region of cucumber mosaic virus RNA4 confers highly competitive activity on heterologous luciferase mRNA in cell-free translation
AU - Kwon, Chang Seob
AU - Paek, Kyung Hee
AU - Chung, Won Il
N1 - Funding Information:
This research was supported partly by Korea Science and Engineering Foundation and partly by the Genetic Engineering Research Fund of the Korea Ministry of Education.
PY - 1998/1/23
Y1 - 1998/1/23
N2 - The cell-free translation of virion RNAs of several tripartite RNA viruses has shown that RNA4, a subgenomic RNA, is more competitive than other virion RNAs. Recently, the 3' untranslated region (UTR) of alfalfa mosaic virus (AMV) RNA4 was identified to be a competitive determinant. In this study, we observed that the RNA4 of cucumber mosaic virus (CMV), another tripartite RNA virus, was also found to be a strong competitor in translational competition among CMV virion RNAs. To identify the competitive determinant of CMV RNA4, we constructed various chimeric luciferase mRNAs containing RNA4 and/or vector-derived UTRs. The relative translations of luciferase-containing mRNA in the presence of a competitor mRNA showed that the 5' UTR, not the 3' UTR, substantially contributed to the highly competitive activity of CMV RNA4.
AB - The cell-free translation of virion RNAs of several tripartite RNA viruses has shown that RNA4, a subgenomic RNA, is more competitive than other virion RNAs. Recently, the 3' untranslated region (UTR) of alfalfa mosaic virus (AMV) RNA4 was identified to be a competitive determinant. In this study, we observed that the RNA4 of cucumber mosaic virus (CMV), another tripartite RNA virus, was also found to be a strong competitor in translational competition among CMV virion RNAs. To identify the competitive determinant of CMV RNA4, we constructed various chimeric luciferase mRNAs containing RNA4 and/or vector-derived UTRs. The relative translations of luciferase-containing mRNA in the presence of a competitor mRNA showed that the 5' UTR, not the 3' UTR, substantially contributed to the highly competitive activity of CMV RNA4.
KW - 5' Untranslated region
KW - Competitive determinant
KW - Cucumber mosaic virus
KW - Translation efficiency
KW - mRNA competition
UR - http://www.scopus.com/inward/record.url?scp=0032559390&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(97)01599-8
DO - 10.1016/S0014-5793(97)01599-8
M3 - Article
C2 - 9475176
AN - SCOPUS:0032559390
SN - 0014-5793
VL - 422
SP - 89
EP - 93
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -