Abstract
The cell-free translation of virion RNAs of several tripartite RNA viruses has shown that RNA4, a subgenomic RNA, is more competitive than other virion RNAs. Recently, the 3' untranslated region (UTR) of alfalfa mosaic virus (AMV) RNA4 was identified to be a competitive determinant. In this study, we observed that the RNA4 of cucumber mosaic virus (CMV), another tripartite RNA virus, was also found to be a strong competitor in translational competition among CMV virion RNAs. To identify the competitive determinant of CMV RNA4, we constructed various chimeric luciferase mRNAs containing RNA4 and/or vector-derived UTRs. The relative translations of luciferase-containing mRNA in the presence of a competitor mRNA showed that the 5' UTR, not the 3' UTR, substantially contributed to the highly competitive activity of CMV RNA4.
Original language | English |
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Pages (from-to) | 89-93 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 422 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1998 Jan 23 |
Bibliographical note
Funding Information:This research was supported partly by Korea Science and Engineering Foundation and partly by the Genetic Engineering Research Fund of the Korea Ministry of Education.
Keywords
- 5' Untranslated region
- Competitive determinant
- Cucumber mosaic virus
- Translation efficiency
- mRNA competition
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology