The analysis of differentially expressed novel transcripts in diapausing and diapause-activated eggs of Bombyx mori

Jae Sam Hwang; Hyun Jeong Go; Tae Won Goo; Eun Young Yun; Kwang Ho Choi; Su Il Seong; Sang Mong Lee; Bong Hee Lee; Iksoo Kim; Taehoon Chun; Seok Woo Kang

doi:10.1002/arch.20057

The analysis of differentially expressed novel transcripts in diapausing and diapause-activated eggs of Bombyx mori

Jae Sam Hwang, Hyun Jeong Go, Tae Won Goo, Eun Young Yun, Kwang Ho Choi, Su Il Seong, Sang Mong Lee, Bong Hee Lee, Iksoo Kim, Taehoon Chun, Seok Woo Kang

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

As an initial step to define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworms, Bombyx mori, mRNA transcripts from maintained and activated diapause eggs were compared with differential expression using cDNA array. Twenty-four individual cDNA transcripts were expressed differentially in a total of 1,468 different cDNAs. Among those clones, mRNA transcript from cytochrome oxidase subunit I (COI), which was detected to be 2-kb transcripts, gradually increased in diapause-activated eggs during early embryogenesis. Further analysis revealed that mRNA transcripts from silkworm COI were highly expressed in testis, fat body, and midgut during the larval stage. These results may indicate that the expression of silkworm COI mRNA is regulated developmentally as well as tissue-specifically.

Original language	English
Pages (from-to)	197-201
Number of pages	5
Journal	Archives of Insect Biochemistry and Physiology
Volume	59
Issue number	4
DOIs	https://doi.org/10.1002/arch.20057
Publication status	Published - 2005 Aug
Externally published	Yes

Keywords

Cytochrome oxidase subunit I
DNA array
Diapause
Embryogenesis
Insects
Silkworm

ASJC Scopus subject areas

Physiology
Biochemistry
Insect Science

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10.1002/arch.20057

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title = "The analysis of differentially expressed novel transcripts in diapausing and diapause-activated eggs of Bombyx mori",

abstract = "As an initial step to define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworms, Bombyx mori, mRNA transcripts from maintained and activated diapause eggs were compared with differential expression using cDNA array. Twenty-four individual cDNA transcripts were expressed differentially in a total of 1,468 different cDNAs. Among those clones, mRNA transcript from cytochrome oxidase subunit I (COI), which was detected to be 2-kb transcripts, gradually increased in diapause-activated eggs during early embryogenesis. Further analysis revealed that mRNA transcripts from silkworm COI were highly expressed in testis, fat body, and midgut during the larval stage. These results may indicate that the expression of silkworm COI mRNA is regulated developmentally as well as tissue-specifically.",

keywords = "Cytochrome oxidase subunit I, DNA array, Diapause, Embryogenesis, Insects, Silkworm",

author = "Hwang, {Jae Sam} and Go, {Hyun Jeong} and Goo, {Tae Won} and Yun, {Eun Young} and Choi, {Kwang Ho} and Seong, {Su Il} and Lee, {Sang Mong} and Lee, {Bong Hee} and Iksoo Kim and Taehoon Chun and Kang, {Seok Woo}",

year = "2005",

month = aug,

doi = "10.1002/arch.20057",

language = "English",

volume = "59",

pages = "197--201",

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AU - Hwang, Jae Sam

AU - Go, Hyun Jeong

AU - Goo, Tae Won

AU - Yun, Eun Young

AU - Choi, Kwang Ho

AU - Seong, Su Il

AU - Lee, Sang Mong

AU - Lee, Bong Hee

AU - Kim, Iksoo

AU - Chun, Taehoon

AU - Kang, Seok Woo

PY - 2005/8

Y1 - 2005/8

N2 - As an initial step to define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworms, Bombyx mori, mRNA transcripts from maintained and activated diapause eggs were compared with differential expression using cDNA array. Twenty-four individual cDNA transcripts were expressed differentially in a total of 1,468 different cDNAs. Among those clones, mRNA transcript from cytochrome oxidase subunit I (COI), which was detected to be 2-kb transcripts, gradually increased in diapause-activated eggs during early embryogenesis. Further analysis revealed that mRNA transcripts from silkworm COI were highly expressed in testis, fat body, and midgut during the larval stage. These results may indicate that the expression of silkworm COI mRNA is regulated developmentally as well as tissue-specifically.

AB - As an initial step to define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworms, Bombyx mori, mRNA transcripts from maintained and activated diapause eggs were compared with differential expression using cDNA array. Twenty-four individual cDNA transcripts were expressed differentially in a total of 1,468 different cDNAs. Among those clones, mRNA transcript from cytochrome oxidase subunit I (COI), which was detected to be 2-kb transcripts, gradually increased in diapause-activated eggs during early embryogenesis. Further analysis revealed that mRNA transcripts from silkworm COI were highly expressed in testis, fat body, and midgut during the larval stage. These results may indicate that the expression of silkworm COI mRNA is regulated developmentally as well as tissue-specifically.

KW - Cytochrome oxidase subunit I

KW - DNA array

KW - Diapause

KW - Embryogenesis

KW - Insects

KW - Silkworm

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ER -

The analysis of differentially expressed novel transcripts in diapausing and diapause-activated eggs of Bombyx mori

Abstract

Keywords

ASJC Scopus subject areas

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