Diospyros kaki L.f., Ebenaceae, have been used since centuries in traditional medicines in many Asian countries as it contains numerous flavonoids. Out of those plenty flavonoids, quercetin-3-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranoside (1) was found to be one of those active components of the plant. In the present study, we confirmed the non-toxicity behavior of 1 on RAW 264.7 macrophage cells and demonstrated its anti-inflammatory property. Our result indicates that 1 treatment significantly controlled the upregulation of the mitogen-activated protein kinase pathway in a dose-dependent manner in the Escherichia coli produced lipopolysaccharide induced infection background. In addition, its treatment significantly reduced the nitric oxide level, and down-regulated the protein expression of TNF-α, PGE2, IL-1β, IL-6, COX-2, and iNOS in lipopolysaccharide stimulated RAW 264.7 macrophage cells. Taken together, our results suggested that 1 efficiently modulates the MAPK signaling pathway and controlled the inflammatory event. Collectively, the result of the study confirmed the potential of 1 as a potent drug candidate and suggested its usage against the inflammatory diseases. [Figure not available: see fulltext.].
|Number of pages||7|
|Journal||Revista Brasileira de Farmacognosia|
|Publication status||Published - 2020 Apr|
- Medicinal plant
- Signaling pathway
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics(all)