The arabidopsis cysteine-rich receptor-like kinase crk36 regulates immunity through interaction with the cytoplasmic kinase BIK1

Dong Sook Lee, Young Cheon Kim, Sun Jae Kwon, Choong Min Ryu, Ohkmae K. Park

    Research output: Contribution to journalArticlepeer-review

    67 Citations (Scopus)

    Abstract

    Receptor-like kinases are important signaling components that regulate a variety of cellular processes. In this study, an Arabidopsis cDNA microarray analysis led to the identification of the cysteine-rich receptor-like kinase CRK36 responsive to the necrotrophic fungal pathogen, Alternaria brassicicola. To determine the function of CRK36 in plant immunity, T-DNA-insertion knockdown (crk36) and overexpressing (CRK36OE) plants were prepared. CRK36OE plants exhibited increased hypersensitive cell death and ROS burst in response to avirulent pathogens. Treatment with a typical pathogen-associated molecular pattern, flg22, markedly induced pattern-triggered immune responses, notably stomatal defense, in CRK36OE plants. The immune responses were weakened in crk36 plants. Protein-protein interaction assays revealed the in vivo association of CRK36, FLS2, and BIK1. CRK36 enhanced flg22-triggered BIK1 phosphorylation, which showed defects with Cys mutations in the DUF26 motifs of CRK36. Disruption of BIK1 and RbohD/RbohF genes further impaired CRK36-mediated stomatal defense. We propose that CRK36, together with BIK1 and NADPH oxidases, may form a positive activation loop that enhances ROS burst and leads to the promotion of stomatal immunity.

    Original languageEnglish
    Article number1856
    JournalFrontiers in Plant Science
    Volume8
    DOIs
    Publication statusPublished - 2017 Oct 27

    Bibliographical note

    Funding Information:
    This work was supported by a Korea University grant, the Next-Generation BioGreen 21 Programs (SSAC, PJ011209; Plant Molecular Breeding Center, PJ011210) through the Rural Development Administration, and a National Research Foundation of Korea (NRF) grant funded by the Korean government (2017R1A2B4009991).

    Publisher Copyright:
    © 2017 Lee, Kim, Kwon, Ryu and Park.

    Keywords

    • Arabidopsis thaliana
    • BiK1
    • CRK36
    • Cysteine-rich receptor-like kinase
    • FLS2
    • NADPH oxidase
    • Pattern-triggered immunity
    • Stomatal immunity

    ASJC Scopus subject areas

    • Plant Science

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