@article{4fe9c7e7cadf48e3b29dddc1bec2d77d,
title = "The chejuenolide biosynthetic gene cluster harboring an iterative trans-AT PKS system in Hahella chejuensis strain MB-1084",
abstract = "Hahella chejuensis MB-1084 is a Gram-negative marine bacterial strain that produces unusual 17-membered carbocyclic tetraenes, chejuenolide A and B. Two fosmid clones responsible for chejuenolide production were identified from the genomic DNA library of the MB-1084 strain. Systematic inactivation of the open reading frames (ORFs) in the sequenced region defines the boundaries of the chejuenolide (che) biosynthetic gene cluster (24.9 kbp) that encodes one non-ribosomal peptide synthase (NRPS)-polyketide synthase (PKS) hybrid protein, three modular PKSs, two PKS domains, and an amine oxidase homolog. Based on the results, we found that the che PKSs have non-canonical features such as trans-AT system and insufficient number of KS domains (five KS domains) for chejuenolide production (requires eight rounds of Claisen condensation reaction). Heterologous expression of the che PKSs in the E. coli BAP1 strain provides strong evidence of the iterative characteristic of the modular PKSs. Additionally, the phylogenetic relatedness of the KS domains of che PKSs and other trans-AT PKSs was analyzed to propose a possible pathway for chejuenolide biosynthesis.",
author = "Ng, {Bee Gek} and Han, {Jae Woo} and Lee, {Dong Wan} and Choi, {Gyung Ja} and Kim, {Beom Seok}",
note = "Funding Information: This work was supported by a Grant of Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2017R1D1A1B03027996). We sincerely thank Professor Piel Jorn from Department of Biology, ETH Zurich for the advice on phylogenetic analysis, Professor Blaine Pfeifer from Department of Chemical and Biological Engineering, State University of New York for providing the E. coli BAP1 strain and Professor Kim Kun Soo from Department of Life Sciences, Sogang University for providing plasmid pKNG101 Funding Information: Acknowledgements This work was supported by a Grant of Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2017R1D1A1B03027996). We sincerely thank Professor Piel J{\"o}rn from Department of Biology, ETH Z{\"u}rich for the advice on phylogenetic analysis, Professor Blaine Pfeifer from Department of Chemical and Biological Engineering, State University of New York for providing the E. coli BAP1 strain and Professor Kim Kun Soo from Department of Life Sciences, Sogang University for providing plasmid pKNG101. Publisher Copyright: {\textcopyright} 2018 The Author(s) [YEAR], under exclusive licence to the Japan Antibiotics Research Association.",
year = "2018",
month = may,
day = "1",
doi = "10.1038/s41429-017-0023-x",
language = "English",
volume = "71",
pages = "495--505",
journal = "Journal of Antibiotics",
issn = "0021-8820",
publisher = "Japan Antibiotics Research Association",
number = "5",
}