The effect of gelatin incorporation into electrospun poly(l-lactide-co-ε-caprolactone) fibers on mechanical properties and cytocompatibility

Jongman Lee, Giyoong Tae, Young Ha Kim, In Su Park, Sang Heon Kim, Soo Hyun Kim

    Research output: Contribution to journalArticlepeer-review

    176 Citations (Scopus)

    Abstract

    Very elastic poly(l-lactide-co-ε-caprolactone) (PLCL) (50:50) copolymer blended with gelatin was electrospun into microfibers from a hexafluoroisopropanol solution. PLCL fiber sheet exhibited the unique soft and flexible behavior while gelatin fiber was hard and brittle. As the gelatin content of PLCL/gelatin fibers increased, Young's modulus was increased, but the elongation was decreased compared to those of PLCL. However, fibers containing 10-30 wt% gelatin demonstrated an enhanced tensile strength with still high elongation to be beneficial for tissue engineering scaffolds. The cytocompatibility of electrospun fiber sheets was evaluated by fibroblasts (NIH-3T3) cell culture. The initial cell adhesion on various fibers after 5 h was somewhat similar, but in the order of PLCL > PLCL70/gelatin30 ≈ PLCL50/gelatin50 > PLCL90/gelatin10 ≈ gelatin > PLCL30/gelatin70. However, the cell proliferation exhibited a completely different and strong dependence on the fiber composition: a very high proliferation rate on PLCL90/gelatin10, followed by PLCL > gelatin > PLCL70/gelatin30. Such an enhanced effect of gelatin, especially at 10 wt% content, on strength and cytocompatibility of PLCL/gelatin fibers would be very preferable for tissue engineering scaffolds.

    Original languageEnglish
    Pages (from-to)1872-1879
    Number of pages8
    JournalBiomaterials
    Volume29
    Issue number12
    DOIs
    Publication statusPublished - 2008 Apr

    Bibliographical note

    Funding Information:
    This work was supported by the Korea Foundation for International Cooperation of Science & Technology through a grant provided by the Korean Ministry of Science & Technology in Italy–Korea Joint Research Projects Program, K20713000010-07B0100-01010.

    Copyright:
    Copyright 2009 Elsevier B.V., All rights reserved.

    Keywords

    • Cytocompatibility
    • Electrospinning
    • Fibroblasts
    • PLCL-gelatin fibers

    ASJC Scopus subject areas

    • Biophysics
    • Bioengineering
    • Ceramics and Composites
    • Biomaterials
    • Mechanics of Materials

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