The effect of low-intensity cold atmospheric plasma jet on photoaging-induced hyperpigmentation in mouse model

Ga Ram Ahn, Hyung Joon Park, Young Gue Koh, Ka Ram Kim, Yu Jin Kim, Jung Ok Lee, Joon Seok, Kwang Ho Yoo, Kyu Back Lee, Beom Joon Kim

Research output: Contribution to journalArticlepeer-review


Background: Cold atmospheric plasma (CAP) produces reactive oxygen/nitrogen species (RONS) in the target and can induce cytoprotective effects by activating hormesis-related pathways when its intensity is in the low range. Objectives: The aim of this study is to evaluate the effect of low-intensified CAP (LICAP) on skin with photoaging-induced hyperpigmentation in an animal model. Methods: Changes in cell viability and RONS production following LICAP treatment were measured. For the in vivo study, 30 hairless mice underwent antecedent photoaging induction followed by the allocated therapy (i.e., LICAP, topical ascorbic acid (AA), or both). During the first 4 weeks of the treatment period (8 weeks), ultraviolet (UV)-B irradiation was concurrently administered. Visual inspection and measurement of the melanin index (MI) were performed to assess the change in skin pigmentation at Weeks 0, 2, 4, 6, and 8. Results: RONS production increased linearly until the saturation point. Cell viability was not significantly affected by LICAP treatment. At Week 8, MI was significantly decreased in every treatment group compared with the values at Week 0 and Week 4. The treatment effect of the concurrent therapy group was superior to that of the LICAP and AA groups. Conclusion: LICAP appears to be a novel modality for photoprotection and pigment reduction in photodamaged skin. LICAP treatment and topical AA application seem to exert a synergistic effect.

Original languageEnglish
Pages (from-to)2799-2809
Number of pages11
JournalJournal of Cosmetic Dermatology
Issue number10
Publication statusPublished - 2023 Oct

Bibliographical note

Publisher Copyright:
© 2023 The Authors. Journal of Cosmetic Dermatology published by Wiley Periodicals LLC.


  • cold atmospheric plasma
  • melanin index
  • photoaging
  • pigmentation
  • redox hormesis

ASJC Scopus subject areas

  • Dermatology


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