@inproceedings{22af47684fee47a79b459b82df74ac86,
title = "The effect of shear stress on transfection of primary neurons using microfluidic platform",
abstract = "This paper describes a novel microfluidic device that was used to investigate the effect of shear stress on transfection efficiency of lipoplex to neurons. The device can be used to simultaneously generate regions with multiple shear stress levels using a single device. When exposed to shear stress, post-mitotic primary rat cortical neurons' transfection rate increased by upto 3-fold when compared to static conventional method. Similar effect was observed with mitotic neuronal cell line NlE-115 where upto 45 % transfection efficiency was achieved with the aid of shear stress. We demonstrated the efficiency of the reversibly binding microfluidic device in executing transfection experiments and corroborated the fact that shear stress is a new parameter to improve non-viral transfection to cells.",
keywords = "Microfluidics, Neuron, Shear stress, Transfection",
author = "Kim, {Hyung Joon} and Byun, {Jae Hwan} and Sudong Kim and Hu, {Qing Ping} and Ryu, {Hyun Ryul} and Yekyuong Cho and Sim, {Sang Jun} and Shin, {Hwa Sung} and Jeon, {Noo Li}",
note = "Copyright: Copyright 2014 Elsevier B.V., All rights reserved.; 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",
year = "2009",
language = "English",
isbn = "9780979806421",
series = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "1913--1915",
booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}