The effect of soluble LAG-3 (CD223) treatment in fetal thymic organ culture

Taehoon Chun; Hyun Jung Byun; Yong Chung Hee; Yong Hoon Chung

doi:10.1023/B:BILE.0000045635.63873.96

The effect of soluble LAG-3 (CD223) treatment in fetal thymic organ culture

Taehoon Chun, Hyun Jung Byun, Yong Chung Hee, Yong Hoon Chung

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Lymphocyte activation gene-3 (LAG-3; CD223) is structurally similar to CD4 and binds to MHC class II with a 100-fold higher affinity than that of CD4. Soluble LAG-3 (sLAG-3Ig) might be useful for immunotherapy by inducing MHC class II-mediated cell activation. A new form of sLAG-3Ig was constructed containing a critical binding site (D1 and D2 region) to MHC class II, combined with a Fc portion of an immunoglobulin γ1. After treatment of sLAG-3Ig in fetal thymic organ culture from DO11.10 transgenic mouse, CD4⁺ T cell precursors were increased in the positive selection but not affected in the negative selection. Further analysis by treating sLAG-3Ig on thymic epithelial cells revealed that CD40 and MHC class II were up-regulated. These results may demonstrate that the treatment of sLAG-3Ig increases the precursor frequency of CD4⁺ T cells by activation of thymic epithelial cells.

Original language	English
Pages (from-to)	1371-1377
Number of pages	7
Journal	Biotechnology letters
Volume	26
Issue number	17
DOIs	https://doi.org/10.1023/B:BILE.0000045635.63873.96
Publication status	Published - 2004 Sept
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank Hae-Ja Lee for technical assistance. This work was supported by Korea Research Foundation Grant (KRF-2002-003-E00057).

Keywords

Co-receptor
Fetal thymic organ culture
Fusion protein
Lymphocyte activation gene-3
T cell precursor
Thymic selection

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1023/B:BILE.0000045635.63873.96

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abstract = "Lymphocyte activation gene-3 (LAG-3; CD223) is structurally similar to CD4 and binds to MHC class II with a 100-fold higher affinity than that of CD4. Soluble LAG-3 (sLAG-3Ig) might be useful for immunotherapy by inducing MHC class II-mediated cell activation. A new form of sLAG-3Ig was constructed containing a critical binding site (D1 and D2 region) to MHC class II, combined with a Fc portion of an immunoglobulin γ1. After treatment of sLAG-3Ig in fetal thymic organ culture from DO11.10 transgenic mouse, CD4+ T cell precursors were increased in the positive selection but not affected in the negative selection. Further analysis by treating sLAG-3Ig on thymic epithelial cells revealed that CD40 and MHC class II were up-regulated. These results may demonstrate that the treatment of sLAG-3Ig increases the precursor frequency of CD4+ T cells by activation of thymic epithelial cells.",

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note = "Funding Information: The authors thank Hae-Ja Lee for technical assistance. This work was supported by Korea Research Foundation Grant (KRF-2002-003-E00057).",

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AU - Hee, Yong Chung

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AB - Lymphocyte activation gene-3 (LAG-3; CD223) is structurally similar to CD4 and binds to MHC class II with a 100-fold higher affinity than that of CD4. Soluble LAG-3 (sLAG-3Ig) might be useful for immunotherapy by inducing MHC class II-mediated cell activation. A new form of sLAG-3Ig was constructed containing a critical binding site (D1 and D2 region) to MHC class II, combined with a Fc portion of an immunoglobulin γ1. After treatment of sLAG-3Ig in fetal thymic organ culture from DO11.10 transgenic mouse, CD4+ T cell precursors were increased in the positive selection but not affected in the negative selection. Further analysis by treating sLAG-3Ig on thymic epithelial cells revealed that CD40 and MHC class II were up-regulated. These results may demonstrate that the treatment of sLAG-3Ig increases the precursor frequency of CD4+ T cells by activation of thymic epithelial cells.

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KW - Thymic selection

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The effect of soluble LAG-3 (CD223) treatment in fetal thymic organ culture

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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