Abstract
Purpose: The aim of this study is to evaluate the cytotoxic and antiproliferating effects of intravenous anesthetics on an mouse fibroblast in vitro cell culture system. Methods: The cells were exposed to the usual clinical plasma concentration of intravenous anesthetics, i.e., midazolam (0.15 μg/ml), propofol (2 μg/ml), remifentanil (2 μg/ml), thiopental (10 μg/ml), for 4, 8, or 24 h. Cell proliferation (n = 6 for each) under intravenous anesthetics was analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) assay. Cytotoxicity (n = 6 for each) of intravenous anesthetics was investigated using a LIVE/DEAD viability assay kit. Results: Intravenous anesthetic exposure time did not affect the proliferation rate of mouse fibroblasts. The cytotoxicity of intravenous anesthetics did not differ in accordance with exposure time. Conclusion: Our results showed that intravenous anesthetics may not affect mouse fibroblast proliferation and viability.
Original language | English |
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Pages (from-to) | 675-681 |
Number of pages | 7 |
Journal | Journal of Anesthesia |
Volume | 26 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2012 Oct |
Keywords
- Fibroblast
- Midazolam
- Propofol
- Remifentanil
- Sedation
- Transplantation
ASJC Scopus subject areas
- Anesthesiology and Pain Medicine