The effects of oxygen and treatments in hypoxic conditions in sh-sy5y cells

Young Duck Cho, Sung Hyuk Choi, Young Hoon Yoon, Jung Youn Kim, Sung Jun Park, Chae Seung Lim

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Many patients are admitted to the emergency department due to trauma. Patients with massive hemorrhage and respiratory failure can fall into hypovolemic shock. Thereafter, oxygen is an essential part of the treatment of trauma patients, but the mechanisms of its effects in the management of trauma patients remain unknown. Therefore, we conducted an experiment to apply hypoxia, hyperoxia, and other treatment with the goal of decreasing hypoxic neuronal cells damage, as reflected by cell survival, apoptosis, hydrogen peroxide (H2O2) production, and hypoxia-inducible factor 1a (HIF) expression in SH-SY5Y cells. Under hypoxic insults, cell survival percentages decreased and apoptosis was seen with increased necrotic cell death. High-pressure oxygen (80% O2) had no effect compared with normal-pressure oxygen (20% O2). After exposure to hypoxia, H2O2 production and levels of HIF significantly increased compared with normoxia. However, when pentoxifylline (PTX), steroid, and hypertonic saline (HTS) were added after exposure to hypoxic conditions, the production of H2O2 and HIF levels significantly decreased in the groups treated with PTX and HTS. That is, the neuroprotective effect of PTX and HTS alleviated the impacts of hypoxic insulted on neuronal cells.

Original languageEnglish
Pages (from-to)449-454
Number of pages6
JournalShock
Volume50
Issue number4
DOIs
Publication statusPublished - 2018

Bibliographical note

Funding Information:
SH-SY5Y cells were plated to a density of 1 ⨯ 106cells/mL in 96 well plates. Cells were cultured under hypoxic condition (1% O2) for 2 h. To assess the Address reprint requests to Sung-Hyuk Choi, MD, PhD, Department of Emergency Medicine, Korea University Medical Center, Guro Hospital, 148 Gurdong-ro, Guro-gu, Seoul 08308, Korea. E-mail: kuedchoi@korea.ac.kr This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (R1522002), and was partially supported by a Korea University Grant. The authors report no conflicts of interest. DOI: 10.1097/SHK.0000000000001041 Copyright © 2017 by the Shock Society effects of oxygen and medicines, 20% oxygen, 80% oxygen, and another treatments (glycerol, PTX, protease inhibitor, steroid, or HTS) were supplied for 2 h each to cells that had undergone hypoxic insult. After incubation for 24 h at 37°C, the resultant SH-SY5Y cells survival was determined using the MTT assay kit (ATCC, Manassas, Va).

Publisher Copyright:
© 2017 by the Shock Society.

Keywords

  • Hydrogen Peroxide
  • Hypertonic Saline
  • Hypoxia
  • Hypoxia-Inducible Factor
  • Pentoxifylline

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

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