TY - JOUR
T1 - The effects of proinflammatory cytokines on the apoptosis of corneal endothelial cells following argon laser iridotomy
AU - Eom, Youngsub
AU - Kwon, Junki
AU - Heo, Jeong Hwa
AU - Yun, Cheolmin
AU - Kang, Su Yeon
AU - Kim, Hyo Myung
AU - Song, Jong Suk
N1 - Funding Information:
None of the authors have any financial or proprietary interest in any material or method mentioned in this paper. Publication of this article was supported by a grant of the Korean Health Technology R&D Project , Ministry of Health & Welfare, Republic of Korea . ( HI13C0055 ).
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - The aim of this study was to evaluate the relationship between the expression of proinflammatory cytokines and the apoptosis of corneal endothelial cells after argon laser iridotomy (ALI). ALI was performed on each quadrant of the iris in the right eye of mice (ALI1 group). Left eyes were used as control group. The levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, and interferon (IFN)-γ in mice eyes were measured, and TUNEL staining was performed 12 h after ALI. Mice in the ALI-Dexa group were pretreated daily with an intraperitoneal injection of dexamethasone for 4 days before undergoing ALI and compared with mice without dexamethasone pretreatment (ALI2 group). Twelve corneas from six rabbits were incubated ex vivo with (n = 6) or without (n = 6) IL-1β. TUNEL staining was performed 24 h after ex vivo incubation. In the mice experiment, the levels of IL-1β, TNF-α, TGF-β, and IFN-γ were increased in the ALI1 group compared to the control group. Although many TUNEL-positive cells were observed in the ALI1 group, those were not detected in the control group. Dexamethasone pretreatment inhibited the increase in the levels of all four proinflammatory cytokines and reduced TUNEL-positive cells. In the rabbit experiment, TUNEL-positive cells were increased in the incubated corneas with IL-1β compared to those without IL-1β. Expression of proinflammatory cytokines following ALI seems to play a role in the apoptosis of corneal endothelial cells after ALI. Dexamethasone pretreatment inhibited increases in proinflammatory cytokines and reduced the apoptosis of corneal endothelial cells.
AB - The aim of this study was to evaluate the relationship between the expression of proinflammatory cytokines and the apoptosis of corneal endothelial cells after argon laser iridotomy (ALI). ALI was performed on each quadrant of the iris in the right eye of mice (ALI1 group). Left eyes were used as control group. The levels of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, and interferon (IFN)-γ in mice eyes were measured, and TUNEL staining was performed 12 h after ALI. Mice in the ALI-Dexa group were pretreated daily with an intraperitoneal injection of dexamethasone for 4 days before undergoing ALI and compared with mice without dexamethasone pretreatment (ALI2 group). Twelve corneas from six rabbits were incubated ex vivo with (n = 6) or without (n = 6) IL-1β. TUNEL staining was performed 24 h after ex vivo incubation. In the mice experiment, the levels of IL-1β, TNF-α, TGF-β, and IFN-γ were increased in the ALI1 group compared to the control group. Although many TUNEL-positive cells were observed in the ALI1 group, those were not detected in the control group. Dexamethasone pretreatment inhibited the increase in the levels of all four proinflammatory cytokines and reduced TUNEL-positive cells. In the rabbit experiment, TUNEL-positive cells were increased in the incubated corneas with IL-1β compared to those without IL-1β. Expression of proinflammatory cytokines following ALI seems to play a role in the apoptosis of corneal endothelial cells after ALI. Dexamethasone pretreatment inhibited increases in proinflammatory cytokines and reduced the apoptosis of corneal endothelial cells.
KW - Apoptosis
KW - Argon laser iridotomy
KW - Corneal decompensation
KW - Corneal endothelial cell
KW - Proinflammatory cytokine
UR - http://www.scopus.com/inward/record.url?scp=84950151747&partnerID=8YFLogxK
U2 - 10.1016/j.exer.2015.11.022
DO - 10.1016/j.exer.2015.11.022
M3 - Article
C2 - 26657139
AN - SCOPUS:84950151747
SN - 0014-4835
VL - 145
SP - 140
EP - 147
JO - Experimental Eye Research
JF - Experimental Eye Research
ER -