The effects of the physical properties of culture substrates on the growth and differentiation of human embryonic stem cells

Sunray Lee; Jihoon Kim; Tae Jung Park; Youngmin Shin; Sang Yup Lee; Yong Mahn Han; Seongman Kang; Hyun Sook Park

doi:10.1016/j.biomaterials.2011.07.058

The effects of the physical properties of culture substrates on the growth and differentiation of human embryonic stem cells

Sunray Lee, Jihoon Kim, Tae Jung Park, Youngmin Shin, Sang Yup Lee, Yong Mahn Han, Seongman Kang, Hyun Sook Park

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The physical factors of cell-culture environment have received little attention despite their anticipated significant role in human embryonic stem cell (hESC) culture optimization. Here we show that hESC culture conditions can be optimized by utilizing polyethylene terephthalate (PET) membranes whose defined pore densities (PDs) determine membrane surface hardness. The PET membranes with 1-4 × 10 ⁶ pores/cm ² (0.291-0.345 GPa) supported the adherence and survival of hESCs without matrix coating. Furthermore, PET membrane with 4 × 10 ⁶ pores/cm ² (0.345 GPa) supported optimal hESC self renewal as well as by the increase in cell proliferation. The expression level and activity of Rho-associated kinase (ROCK) were specifically down-regulated in hESCs cultured on the optimal PET membrane. We suggest that PET membranes of a defined PD/hardness provide an excellent culture substrate for the maintenance of uniform and undifferentiated hESCs.

Original language	English
Pages (from-to)	8816-8829
Number of pages	14
Journal	Biomaterials
Volume	32
Issue number	34
DOIs	https://doi.org/10.1016/j.biomaterials.2011.07.058
Publication status	Published - 2011 Dec

Keywords

Embryonic stem cells
Hardness
Polymer membrane
Pore density
Rho family GTPase

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2011.07.058

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title = "The effects of the physical properties of culture substrates on the growth and differentiation of human embryonic stem cells",

abstract = "The physical factors of cell-culture environment have received little attention despite their anticipated significant role in human embryonic stem cell (hESC) culture optimization. Here we show that hESC culture conditions can be optimized by utilizing polyethylene terephthalate (PET) membranes whose defined pore densities (PDs) determine membrane surface hardness. The PET membranes with 1-4 × 10 6 pores/cm 2 (0.291-0.345 GPa) supported the adherence and survival of hESCs without matrix coating. Furthermore, PET membrane with 4 × 10 6 pores/cm 2 (0.345 GPa) supported optimal hESC self renewal as well as by the increase in cell proliferation. The expression level and activity of Rho-associated kinase (ROCK) were specifically down-regulated in hESCs cultured on the optimal PET membrane. We suggest that PET membranes of a defined PD/hardness provide an excellent culture substrate for the maintenance of uniform and undifferentiated hESCs.",

keywords = "Embryonic stem cells, Hardness, Polymer membrane, Pore density, Rho family GTPase",

author = "Sunray Lee and Jihoon Kim and Park, {Tae Jung} and Youngmin Shin and Lee, {Sang Yup} and Han, {Yong Mahn} and Seongman Kang and Park, {Hyun Sook}",

note = "Funding Information: This work was supported by a grant ( SC2250 ) from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology in Republic of Korea . The authors are grateful for the expert advice on the manuscript by Mahendra Rao (Invitrogen Co.), and for the sincere review of the manuscript by Dr Pablo Menendez, Andalusian Stem Cell Bank, Spain. The authors also thank Millipore Inc. for providing MilliCell membranes. ",

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AU - Han, Yong Mahn

AU - Kang, Seongman

AU - Park, Hyun Sook

N1 - Funding Information: This work was supported by a grant ( SC2250 ) from the Stem Cell Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology in Republic of Korea . The authors are grateful for the expert advice on the manuscript by Mahendra Rao (Invitrogen Co.), and for the sincere review of the manuscript by Dr Pablo Menendez, Andalusian Stem Cell Bank, Spain. The authors also thank Millipore Inc. for providing MilliCell membranes.

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The effects of the physical properties of culture substrates on the growth and differentiation of human embryonic stem cells

Abstract

Keywords

ASJC Scopus subject areas

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