Abstract
Small non-coding RNAs regulate gene expression in a sequence-specific manner. In Drosophila, Dicer-2 (Dcr-2) functions in the biogenesis of endogenous small interfering RNAs (endo-siRNAs). We identified 21 distinct proteins that exhibited a ≥1.5-fold change as a consequence of loss of dcr-2 function. Most of these were metabolic genes implicated in stress resistance and aging. dcr-2 Mutants had reduced lifespan and were hypersensitive to oxidative, endoplasmic reticulum, starvation, and cold stresses. Furthermore, loss of dcr-2 function led to abnormal lipid and carbohydrate metabolism. Our results suggest roles for the endo-siRNA pathway in metabolic regulation and defense against stress and aging in Drosophila.
Original language | English |
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Pages (from-to) | 3079-3085 |
Number of pages | 7 |
Journal | FEBS Letters |
Volume | 585 |
Issue number | 19 |
DOIs | |
Publication status | Published - 2011 Oct 3 |
Bibliographical note
Funding Information:We thank R. Carthew, G. Hannon, Q. Liu, M. Siomi, and A. Lambertsson for antibodies and Drosophila strains, and the Developmental Studies Hybridoma Bank for antibodies. D.H. Lim was supported by a Hi Seoul Science/Humanities Fellowship from Seoul Scholarship Foundation. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (KRF-2008-313-C00640 to Y.S. Lee), by Brain Korea 21 Project from the Ministry of Education, Science, and Technology of Korea (to Korea University), and by KOBIC Research Support Program .
Keywords
- Dicer-2
- Drosophila
- Endogenous siRNA
- Lifespan
- Metabolic homeostasis
- Stress response
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology