The enhanced IL-18 production by UVB irradiation requires ROI and AP-1 signaling in human keratinocyte cell line (HaCaT)

Daeho Cho, Jae Seung Kang, Jong Hoon Park, Young In Kim, Eunsil Hahm, Junechul Lee, Yoolhee Yang, Junho Jeon, Hyunkeun Song, Hyunjeong Park, Taesung Kim, Saic Pang, Chul Woo Kim, Young Il Hwang, Wang Jae Lee

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


Based on our recent observation that enhanced IL-18 expression positively correlates with malignant skin tumors, such as SCC and melanoma, we examined the possible role of UVB, known to be associated with skin cancer development, in the enhancement of IL-18 production using primary human epidermal keratinocytes and human keratinocyte cell line HaCaT. After cells were exposed to UVB irradiation in vitro, IL-18 production was examined by Northern blot analysis and ELISA, and it was found that IL-18 production is enhanced by UVB irradiation in a dose- and time-dependent manner. In addition, we confirmed that it is functionally active form of IL-18 using the inhibitor of caspase-1. The effect of UVB irradiation was blocked by antioxidant, N-acetyl-L-cysteine (NAC), which suggested the involvement of reactive oxygen intermediates (ROI) in the signal transduction of UVB irradiation-enhanced IL-18 synthesis. We also found that UVB irradiation increased AP-1 binding activity by using EMSA with AP-1-specific oligonucleotide. Furthermore, inhibitors of UVB-induced AP-1 activity, such as PD98059, blocked enhanced IL-18 production, indicating that AP-1 activation is required for UVB-induced IL-18 production. Taken together, our results suggest that UVB irradiation-enhanced IL-18 production is selectively mediated through the generation of ROI and the activation of AP-1.

Original languageEnglish
Pages (from-to)289-295
Number of pages7
JournalBiochemical and biophysical research communications
Issue number2
Publication statusPublished - 2002
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by Korea Research Foundation Grant (KRF-2002-005-C00015).


  • AP-1 element
  • IL-18
  • Keratinocyte
  • UVB

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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