The expression of PHO92 is regulated by Gcr1, and Pho92 is involved in glucose metabolism in Saccharomyces cerevisiae

Hyun Jun Kang; Miwha Chang; Chang Min Kang; Yong Sung Park; Bong June Yoon; Tae Hyoung Kim; Cheol Won Yun

doi:10.1007/s00294-014-0430-5

The expression of PHO92 is regulated by Gcr1, and Pho92 is involved in glucose metabolism in Saccharomyces cerevisiae

Hyun Jun Kang, Miwha Chang, Chang Min Kang, Yong Sung Park, Bong June Yoon, Tae Hyoung Kim, Cheol Won Yun

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Ydr374c (Pho92) contains a YTH domain in its C-terminal region and is a human YTHDF2 homologue. Previously, we reported that Pho92 regulates phosphate metabolism by regulating PHO4 mRNA stability. In this study, we found that growth of the ∆pho92 strain on SG media was slower than that of the wild type and that PHO92 expression was up-regulated by non-fermentable carbon sources, such as ethanol and glycerol, but not by fermentable carbon sources. Furthermore, two conserved Gcr1-binding regions were identified in the upstream, untranslated region of PHO92. Gcr1 is an important factor involved in the coordinated regulation of glycolytic gene expression. Mutation of two Gcr1-binding sites of the PHO92 upstream region resulted in a growth defect on SD media. Finally, mutagenesis of the Gcr1-binding sites of the PHO92 upstream region and deletion of GCR1 resulted in up-regulation of PHO92, and this resulted from inhibition of PHO4 mRNA degradation. Based on these results, we suggest that Gcr1 regulates the expression of PHO92, and Pho92 is involved in glucose metabolism.

Original language	English
Pages (from-to)	247-253
Number of pages	7
Journal	Current Genetics
Volume	60
Issue number	4
DOIs	https://doi.org/10.1007/s00294-014-0430-5
Publication status	Published - 2014 Nov

Bibliographical note

Funding Information:
Acknowledgments This work was supported by a Korea University Grant and a grant from the National Research Foundation of Korea (NRF) (No. 2012-0007043).

Publisher Copyright:
© 2014, Springer-Verlag Berlin Heidelberg.

Keywords

Gcr1
Pho4
Pho92
Phosphate
S. cerevisiae
YTH domain

ASJC Scopus subject areas

Genetics

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10.1007/s00294-014-0430-5

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title = "The expression of PHO92 is regulated by Gcr1, and Pho92 is involved in glucose metabolism in Saccharomyces cerevisiae",

abstract = "Ydr374c (Pho92) contains a YTH domain in its C-terminal region and is a human YTHDF2 homologue. Previously, we reported that Pho92 regulates phosphate metabolism by regulating PHO4 mRNA stability. In this study, we found that growth of the ∆pho92 strain on SG media was slower than that of the wild type and that PHO92 expression was up-regulated by non-fermentable carbon sources, such as ethanol and glycerol, but not by fermentable carbon sources. Furthermore, two conserved Gcr1-binding regions were identified in the upstream, untranslated region of PHO92. Gcr1 is an important factor involved in the coordinated regulation of glycolytic gene expression. Mutation of two Gcr1-binding sites of the PHO92 upstream region resulted in a growth defect on SD media. Finally, mutagenesis of the Gcr1-binding sites of the PHO92 upstream region and deletion of GCR1 resulted in up-regulation of PHO92, and this resulted from inhibition of PHO4 mRNA degradation. Based on these results, we suggest that Gcr1 regulates the expression of PHO92, and Pho92 is involved in glucose metabolism.",

keywords = "Gcr1, Pho4, Pho92, Phosphate, S. cerevisiae, YTH domain",

author = "Kang, {Hyun Jun} and Miwha Chang and Kang, {Chang Min} and Park, {Yong Sung} and Yoon, {Bong June} and Kim, {Tae Hyoung} and Yun, {Cheol Won}",

note = "Funding Information: Acknowledgments This work was supported by a Korea University Grant and a grant from the National Research Foundation of Korea (NRF) (No. 2012-0007043). Publisher Copyright: {\textcopyright} 2014, Springer-Verlag Berlin Heidelberg.",

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doi = "10.1007/s00294-014-0430-5",

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AU - Kang, Hyun Jun

AU - Chang, Miwha

AU - Kang, Chang Min

AU - Park, Yong Sung

AU - Yoon, Bong June

AU - Kim, Tae Hyoung

AU - Yun, Cheol Won

N1 - Funding Information: Acknowledgments This work was supported by a Korea University Grant and a grant from the National Research Foundation of Korea (NRF) (No. 2012-0007043). Publisher Copyright: © 2014, Springer-Verlag Berlin Heidelberg.

PY - 2014/11

Y1 - 2014/11

N2 - Ydr374c (Pho92) contains a YTH domain in its C-terminal region and is a human YTHDF2 homologue. Previously, we reported that Pho92 regulates phosphate metabolism by regulating PHO4 mRNA stability. In this study, we found that growth of the ∆pho92 strain on SG media was slower than that of the wild type and that PHO92 expression was up-regulated by non-fermentable carbon sources, such as ethanol and glycerol, but not by fermentable carbon sources. Furthermore, two conserved Gcr1-binding regions were identified in the upstream, untranslated region of PHO92. Gcr1 is an important factor involved in the coordinated regulation of glycolytic gene expression. Mutation of two Gcr1-binding sites of the PHO92 upstream region resulted in a growth defect on SD media. Finally, mutagenesis of the Gcr1-binding sites of the PHO92 upstream region and deletion of GCR1 resulted in up-regulation of PHO92, and this resulted from inhibition of PHO4 mRNA degradation. Based on these results, we suggest that Gcr1 regulates the expression of PHO92, and Pho92 is involved in glucose metabolism.

AB - Ydr374c (Pho92) contains a YTH domain in its C-terminal region and is a human YTHDF2 homologue. Previously, we reported that Pho92 regulates phosphate metabolism by regulating PHO4 mRNA stability. In this study, we found that growth of the ∆pho92 strain on SG media was slower than that of the wild type and that PHO92 expression was up-regulated by non-fermentable carbon sources, such as ethanol and glycerol, but not by fermentable carbon sources. Furthermore, two conserved Gcr1-binding regions were identified in the upstream, untranslated region of PHO92. Gcr1 is an important factor involved in the coordinated regulation of glycolytic gene expression. Mutation of two Gcr1-binding sites of the PHO92 upstream region resulted in a growth defect on SD media. Finally, mutagenesis of the Gcr1-binding sites of the PHO92 upstream region and deletion of GCR1 resulted in up-regulation of PHO92, and this resulted from inhibition of PHO4 mRNA degradation. Based on these results, we suggest that Gcr1 regulates the expression of PHO92, and Pho92 is involved in glucose metabolism.

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The expression of PHO92 is regulated by Gcr1, and Pho92 is involved in glucose metabolism in Saccharomyces cerevisiae

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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