The first bacterial β-1,6-endoglucanase from Saccharophagus degradans 2-40T for the hydrolysis of pustulan and laminarin

Damao Wang, Do Hyoung Kim, Eun Ju Yun, Yong Cheol Park, Jin Ho Seo, Kyoung Heon Kim

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


β-1,6-glucan is a polysaccharide found in brown macroalgae and fungal cell walls. In this study, a β-1,6-endoglucanase gene from Saccharophagus degradans 2-40T, gly30B, was cloned and overexpressed in Escherichia coli. Gly30B, which belongs to the glycoside hydrolase family 30 (GH30), was found to possess β-1,6-endoglucanase activity by hydrolyzing β-1,6-glycosidic linkages of pustulan (β-1,6-glucan derived from fungal cell walls) and laminarin (β-1,3-glucan with β-1,6-branchings, derived from brown macroalgae) to produce gentiobiose and glucose as the final products. The optimal pH and temperature for Gly30B activity were found to be pH 7.0 and 40 °C, respectively. The kinetic constants of Gly30B, Vmax, KM, and kcat were determined to be 153.8 U/mg protein, 24.2 g/L, and 135.6 s−1 for pustulan and 32.8 U/mg protein, 100.8 g/L, and 28.9 s−1 for laminarin, respectively. To our knowledge, Gly30B is the first β-1,6-endoglucanase characterized from bacteria. Gly30B can be used to hydrolyze β-1,6-glucans of brown algae or fungal cell walls for producing gentiobiose as a high-value sugar and glucose as a fermentable sugar.

Original languageEnglish
Pages (from-to)197-204
Number of pages8
JournalApplied Microbiology and Biotechnology
Issue number1
Publication statusPublished - 2017 Jan 1

Bibliographical note

Funding Information:
This work was financially supported by the Advanced Biomass R&D Center of Korea (2011-0031359), funded by the Korean Government (MSIP). Experiments were carried out using the facilities of the Institute of Biomedical Science and Food Safety at the Food Safety Hall, Korea University.

Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.


  • GH30
  • Hydrolysis
  • Saccharophagus degradans 2-40
  • β-1,6-endoglucanase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


Dive into the research topics of 'The first bacterial β-1,6-endoglucanase from Saccharophagus degradans 2-40T for the hydrolysis of pustulan and laminarin'. Together they form a unique fingerprint.

Cite this