Abstract
The gene aeg-46.5, which is expressed under anaerobic condition, has putative triple -10 regions and four transcription start sites. The mRNA transcription level and its start point change depending on the aerobic/anaerobic growth condition. RNA polymerase and its regulatory proteins must choose which of three -10 region to use. The putative triple 10 region was mutated to make only one of them function with consensus -10 region sequence (TATAAT) and the other two as non-functional region. The results show that the second and third -10 regions are used for the aerobic/anaerobic expression. The third -10 region is responsible for the high aerobic to anaerobic switch ratio. This suggests that only the last two of the putative triple -10 region have functions on aeg-46.5 gene expression switch control. The phenotype of the mutated promoter was tested in the wild type cell and narL- cell. The results indicate that the control by NarL is independent from the selection of -10 region. The expression patterns on multi-copy plasmids and on single-copy chromosome were compared. These results show that the aerobic/anaerobic switch control of aeg-46.5 is through the choice of -10 region. The mechanism of choosing different -10 region remains to be seen.
Original language | English |
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Pages (from-to) | 903-908 |
Number of pages | 6 |
Journal | Bulletin of the Korean Chemical Society |
Volume | 22 |
Issue number | 8 |
Publication status | Published - 2001 Aug 20 |
Keywords
- Aerobic-anaerobic switch
- Multiple Pribnow Box
- NarL/NarP regulatory system
- aeg-46.5 gene
ASJC Scopus subject areas
- Chemistry(all)