The genes encoding geranylgeranyl transferase α-subunit and transglutaminase 1 are very closely linked but not functionally related in terminally differentiating keratinocytes

Hae Jun Song; Antonello Rossi; Roberta Ceci; In Gyu Kim; Mario A. Anzano; Shyh Ing Jang; Vincenzo De Laurenzi; Peter M. Steinert

doi:10.1006/bbrc.1997.6717

The genes encoding geranylgeranyl transferase α-subunit and transglutaminase 1 are very closely linked but not functionally related in terminally differentiating keratinocytes

Hae Jun Song, Antonello Rossi, Roberta Ceci, In Gyu Kim, Mario A. Anzano, Shyh Ing Jang, Vincenzo De Laurenzi, Peter M. Steinert

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We report here the entire exon/intron structure of the gene encoding the α-subunit of human Rab geranylgeranyl transferase (RABGGTA) gene, which is positioned in a tandem head-to-tail arrangement with the transglutaminase 1 (TGM1) gene, and its polyadenylation signal sequence is located just 2.3 kbp upstream of the capsite of TGM1. Even though TGM1 and RABGGTA have different functions, their close localization raised the question as to whether they are functionally related in the epidermis. To address this question, we have studied the expression of the two genes by RT-PCR in normal human epidermal keratinocytes cultured under various differentiation conditions. While the expression of the TGM1 gene is markedly affected by the calcium concentration of the medium, all trans retinoic acid, vitamin D₃, and TPA treatment, the expression of the RAB-GGTA gene was unaffected by these reagents. Taken together, even though these two genes are unusually closely linked, they are not functionally related in the terminal differentiation program of epidermal keratinocytes.

Original language	English
Pages (from-to)	10-14
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	235
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1997.6717
Publication status	Published - 1997 Jun 9
Externally published	Yes

Bibliographical note

Funding Information:
We thank Drs. J. Andreoli, T. Kartasova, N. Markova, and G. Rogers for their valuable comments and advice and Ms. C. Coticchia for synthesis of the oligonucleotides used in this work. This work was supported in part by a grant (E413) from TELETON (to V.D.L.).

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1997.6717

Cite this

Song, H. J., Rossi, A., Ceci, R., Kim, I. G., Anzano, M. A., Jang, S. I., De Laurenzi, V., & Steinert, P. M. (1997). The genes encoding geranylgeranyl transferase α-subunit and transglutaminase 1 are very closely linked but not functionally related in terminally differentiating keratinocytes. Biochemical and biophysical research communications, 235(1), 10-14. https://doi.org/10.1006/bbrc.1997.6717

The genes encoding geranylgeranyl transferase α-subunit and transglutaminase 1 are very closely linked but not functionally related in terminally differentiating keratinocytes. / Song, Hae Jun; Rossi, Antonello; Ceci, Roberta et al.
In: Biochemical and biophysical research communications, Vol. 235, No. 1, 09.06.1997, p. 10-14.

Research output: Contribution to journal › Article › peer-review

Song, HJ, Rossi, A, Ceci, R, Kim, IG, Anzano, MA, Jang, SI, De Laurenzi, V & Steinert, PM 1997, 'The genes encoding geranylgeranyl transferase α-subunit and transglutaminase 1 are very closely linked but not functionally related in terminally differentiating keratinocytes', Biochemical and biophysical research communications, vol. 235, no. 1, pp. 10-14. https://doi.org/10.1006/bbrc.1997.6717

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abstract = "We report here the entire exon/intron structure of the gene encoding the α-subunit of human Rab geranylgeranyl transferase (RABGGTA) gene, which is positioned in a tandem head-to-tail arrangement with the transglutaminase 1 (TGM1) gene, and its polyadenylation signal sequence is located just 2.3 kbp upstream of the capsite of TGM1. Even though TGM1 and RABGGTA have different functions, their close localization raised the question as to whether they are functionally related in the epidermis. To address this question, we have studied the expression of the two genes by RT-PCR in normal human epidermal keratinocytes cultured under various differentiation conditions. While the expression of the TGM1 gene is markedly affected by the calcium concentration of the medium, all trans retinoic acid, vitamin D3, and TPA treatment, the expression of the RAB-GGTA gene was unaffected by these reagents. Taken together, even though these two genes are unusually closely linked, they are not functionally related in the terminal differentiation program of epidermal keratinocytes.",

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N2 - We report here the entire exon/intron structure of the gene encoding the α-subunit of human Rab geranylgeranyl transferase (RABGGTA) gene, which is positioned in a tandem head-to-tail arrangement with the transglutaminase 1 (TGM1) gene, and its polyadenylation signal sequence is located just 2.3 kbp upstream of the capsite of TGM1. Even though TGM1 and RABGGTA have different functions, their close localization raised the question as to whether they are functionally related in the epidermis. To address this question, we have studied the expression of the two genes by RT-PCR in normal human epidermal keratinocytes cultured under various differentiation conditions. While the expression of the TGM1 gene is markedly affected by the calcium concentration of the medium, all trans retinoic acid, vitamin D3, and TPA treatment, the expression of the RAB-GGTA gene was unaffected by these reagents. Taken together, even though these two genes are unusually closely linked, they are not functionally related in the terminal differentiation program of epidermal keratinocytes.

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The genes encoding geranylgeranyl transferase α-subunit and transglutaminase 1 are very closely linked but not functionally related in terminally differentiating keratinocytes

Abstract

Bibliographical note

ASJC Scopus subject areas

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