The hydrolysate of barley straw containing inhibitors can be used to produce cephalosporin C by solvent extraction using ethyl acetate

Sung Bong Kim; Hah Young Yoo; Jun Seok Kim; Seung Wook Kim

doi:10.1016/j.procbio.2014.10.007

The hydrolysate of barley straw containing inhibitors can be used to produce cephalosporin C by solvent extraction using ethyl acetate

Sung Bong Kim, Hah Young Yoo, Jun Seok Kim, Seung Wook Kim

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

When the solvent extraction of the hydrolysate from barley straw was performed using ethyl acetate (EA), the logarithm of the partition coefficient (log P) of the phenols and furans for EA was found to be more than 1.00, which means that more than 90% of the inhibitors were removed from the hydrolysate layer. Cephalosporin C (CPC) was produced from the hydrolysate of dilute acid pretreatment (DAP) by Acremonium chrysogenum M35. A. chrysogenum M35 was cultured using the hydrolysate and the amount of CPC produced was found to be 10.35 g/L at 144 h. Also, the dry cell weight was about 101.5 g/L at 120 h. The utilization of the hydrolysate for CPC production was effective and the solvent extraction method for the removal of inhibitory substances could contribute to the biorefinery process.

Original language	English
Pages (from-to)	2203-2206
Number of pages	4
Journal	Process Biochemistry
Volume	49
Issue number	12
DOIs	https://doi.org/10.1016/j.procbio.2014.10.007
Publication status	Published - 2014 Dec

Bibliographical note

Funding Information:
This study was supported by the Advanced Biomass R&D Center (ABC-2011-0031360) of the Global Frontier Project funded by the Ministry of Science, ICT and Future Planning of Korea .

Publisher Copyright:
© 2014 Elsevier Ltd. All rights reserved.

Keywords

Dilute acid pretreatment
Inhibitory substances
Partition coefficient
Solvent extraction
Xylose fermentation

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.procbio.2014.10.007

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title = "The hydrolysate of barley straw containing inhibitors can be used to produce cephalosporin C by solvent extraction using ethyl acetate",

abstract = "When the solvent extraction of the hydrolysate from barley straw was performed using ethyl acetate (EA), the logarithm of the partition coefficient (log P) of the phenols and furans for EA was found to be more than 1.00, which means that more than 90% of the inhibitors were removed from the hydrolysate layer. Cephalosporin C (CPC) was produced from the hydrolysate of dilute acid pretreatment (DAP) by Acremonium chrysogenum M35. A. chrysogenum M35 was cultured using the hydrolysate and the amount of CPC produced was found to be 10.35 g/L at 144 h. Also, the dry cell weight was about 101.5 g/L at 120 h. The utilization of the hydrolysate for CPC production was effective and the solvent extraction method for the removal of inhibitory substances could contribute to the biorefinery process.",

keywords = "Dilute acid pretreatment, Inhibitory substances, Partition coefficient, Solvent extraction, Xylose fermentation",

author = "Kim, {Sung Bong} and Yoo, {Hah Young} and Kim, {Jun Seok} and Kim, {Seung Wook}",

note = "Funding Information: This study was supported by the Advanced Biomass R&D Center (ABC-2011-0031360) of the Global Frontier Project funded by the Ministry of Science, ICT and Future Planning of Korea . Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd. All rights reserved.",

year = "2014",

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AU - Kim, Jun Seok

AU - Kim, Seung Wook

N1 - Funding Information: This study was supported by the Advanced Biomass R&D Center (ABC-2011-0031360) of the Global Frontier Project funded by the Ministry of Science, ICT and Future Planning of Korea . Publisher Copyright: © 2014 Elsevier Ltd. All rights reserved.

PY - 2014/12

Y1 - 2014/12

N2 - When the solvent extraction of the hydrolysate from barley straw was performed using ethyl acetate (EA), the logarithm of the partition coefficient (log P) of the phenols and furans for EA was found to be more than 1.00, which means that more than 90% of the inhibitors were removed from the hydrolysate layer. Cephalosporin C (CPC) was produced from the hydrolysate of dilute acid pretreatment (DAP) by Acremonium chrysogenum M35. A. chrysogenum M35 was cultured using the hydrolysate and the amount of CPC produced was found to be 10.35 g/L at 144 h. Also, the dry cell weight was about 101.5 g/L at 120 h. The utilization of the hydrolysate for CPC production was effective and the solvent extraction method for the removal of inhibitory substances could contribute to the biorefinery process.

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The hydrolysate of barley straw containing inhibitors can be used to produce cephalosporin C by solvent extraction using ethyl acetate

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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