TY - JOUR
T1 - The identification of antigenic proteins
T2 - 14-3-3 protein and propionyl-CoA carboxylase in Clonorchis sinensis
AU - Lee, Myoung Ro
AU - Kim, Yu Jung
AU - Kim, Dae Won
AU - Yoo, Won Gi
AU - Cho, Shin Hyeong
AU - Hwang, Kwang Yeon
AU - Ju, Jung Won
AU - Lee, Won Ja
N1 - Funding Information:
This work was supported by a grant from the Pathogenic Proteome Management Program of the National Institute of Health, Ministry of Health and Welfare, Republic of Korea (NIH 48004847-300 ).
PY - 2012/3
Y1 - 2012/3
N2 - Clonorchis sinensis, the causative agent of clonorchiasis, is widespread in East and Southeast Asia, including China, Vietnam and the Republic of Korea. We identified antigenic proteins from adult C. sinensis liver flukes using immunoproteomic analysis. In this study, we found 23 candidate antigenic proteins with a pI in the range of 5.4-6.2 in total lysates of C. sinensis. The antigenic protein spots reacted against sera from clonorchiasis patients and were identified as cysteine proteases, glutathione transferases, gelsolin, propionyl-CoA carboxylase (PCC), prohibitin and 14-3-3 protein (14-3-3) using LC-coupled ESI-MS/MS and an EST database for C. sinensis. PCC and 14-3-3 were identified for the first time as serological antigens for the diagnosis of C. sinensis. To validate the antigenicity of PCC and 14-3-3, recombinant proteins were immunoblotted with sera from clonorchiasis patients. The structural, functional and immunological characteristics of the putative amino acid sequence were predicted by bioinformatics analysis. Our novel finding will contribute to the development of diagnostics for clonorchiasis. These results suggest that immunoproteomic approaches are valuable tools to identify antigens that could be used as targets for effective parasitic infection control strategies.
AB - Clonorchis sinensis, the causative agent of clonorchiasis, is widespread in East and Southeast Asia, including China, Vietnam and the Republic of Korea. We identified antigenic proteins from adult C. sinensis liver flukes using immunoproteomic analysis. In this study, we found 23 candidate antigenic proteins with a pI in the range of 5.4-6.2 in total lysates of C. sinensis. The antigenic protein spots reacted against sera from clonorchiasis patients and were identified as cysteine proteases, glutathione transferases, gelsolin, propionyl-CoA carboxylase (PCC), prohibitin and 14-3-3 protein (14-3-3) using LC-coupled ESI-MS/MS and an EST database for C. sinensis. PCC and 14-3-3 were identified for the first time as serological antigens for the diagnosis of C. sinensis. To validate the antigenicity of PCC and 14-3-3, recombinant proteins were immunoblotted with sera from clonorchiasis patients. The structural, functional and immunological characteristics of the putative amino acid sequence were predicted by bioinformatics analysis. Our novel finding will contribute to the development of diagnostics for clonorchiasis. These results suggest that immunoproteomic approaches are valuable tools to identify antigens that could be used as targets for effective parasitic infection control strategies.
KW - Antigen
KW - Clonorchis sinensis
KW - Two-dimensional gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=84856708038&partnerID=8YFLogxK
U2 - 10.1016/j.molbiopara.2011.11.006
DO - 10.1016/j.molbiopara.2011.11.006
M3 - Article
C2 - 22119288
AN - SCOPUS:84856708038
SN - 0166-6851
VL - 182
SP - 1
EP - 6
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1-2
ER -