The identification of antigenic proteins: 14-3-3 protein and propionyl-CoA carboxylase in Clonorchis sinensis

Myoung Ro Lee; Yu Jung Kim; Dae Won Kim; Won Gi Yoo; Shin Hyeong Cho; Kwang Yeon Hwang; Jung Won Ju; Won Ja Lee

doi:10.1016/j.molbiopara.2011.11.006

The identification of antigenic proteins: 14-3-3 protein and propionyl-CoA carboxylase in Clonorchis sinensis

Myoung Ro Lee, Yu Jung Kim, Dae Won Kim, Won Gi Yoo, Shin Hyeong Cho, Kwang Yeon Hwang, Jung Won Ju, Won Ja Lee

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Clonorchis sinensis, the causative agent of clonorchiasis, is widespread in East and Southeast Asia, including China, Vietnam and the Republic of Korea. We identified antigenic proteins from adult C. sinensis liver flukes using immunoproteomic analysis. In this study, we found 23 candidate antigenic proteins with a pI in the range of 5.4-6.2 in total lysates of C. sinensis. The antigenic protein spots reacted against sera from clonorchiasis patients and were identified as cysteine proteases, glutathione transferases, gelsolin, propionyl-CoA carboxylase (PCC), prohibitin and 14-3-3 protein (14-3-3) using LC-coupled ESI-MS/MS and an EST database for C. sinensis. PCC and 14-3-3 were identified for the first time as serological antigens for the diagnosis of C. sinensis. To validate the antigenicity of PCC and 14-3-3, recombinant proteins were immunoblotted with sera from clonorchiasis patients. The structural, functional and immunological characteristics of the putative amino acid sequence were predicted by bioinformatics analysis. Our novel finding will contribute to the development of diagnostics for clonorchiasis. These results suggest that immunoproteomic approaches are valuable tools to identify antigens that could be used as targets for effective parasitic infection control strategies.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Molecular and Biochemical Parasitology
Volume	182
Issue number	1-2
DOIs	https://doi.org/10.1016/j.molbiopara.2011.11.006
Publication status	Published - 2012 Mar

Bibliographical note

Funding Information:
This work was supported by a grant from the Pathogenic Proteome Management Program of the National Institute of Health, Ministry of Health and Welfare, Republic of Korea (NIH 48004847-300 ).

Keywords

Antigen
Clonorchis sinensis
Two-dimensional gel electrophoresis

ASJC Scopus subject areas

Parasitology
Molecular Biology

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10.1016/j.molbiopara.2011.11.006

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title = "The identification of antigenic proteins: 14-3-3 protein and propionyl-CoA carboxylase in Clonorchis sinensis",

abstract = "Clonorchis sinensis, the causative agent of clonorchiasis, is widespread in East and Southeast Asia, including China, Vietnam and the Republic of Korea. We identified antigenic proteins from adult C. sinensis liver flukes using immunoproteomic analysis. In this study, we found 23 candidate antigenic proteins with a pI in the range of 5.4-6.2 in total lysates of C. sinensis. The antigenic protein spots reacted against sera from clonorchiasis patients and were identified as cysteine proteases, glutathione transferases, gelsolin, propionyl-CoA carboxylase (PCC), prohibitin and 14-3-3 protein (14-3-3) using LC-coupled ESI-MS/MS and an EST database for C. sinensis. PCC and 14-3-3 were identified for the first time as serological antigens for the diagnosis of C. sinensis. To validate the antigenicity of PCC and 14-3-3, recombinant proteins were immunoblotted with sera from clonorchiasis patients. The structural, functional and immunological characteristics of the putative amino acid sequence were predicted by bioinformatics analysis. Our novel finding will contribute to the development of diagnostics for clonorchiasis. These results suggest that immunoproteomic approaches are valuable tools to identify antigens that could be used as targets for effective parasitic infection control strategies.",

keywords = "Antigen, Clonorchis sinensis, Two-dimensional gel electrophoresis",

author = "Lee, {Myoung Ro} and Kim, {Yu Jung} and Kim, {Dae Won} and Yoo, {Won Gi} and Cho, {Shin Hyeong} and Hwang, {Kwang Yeon} and Ju, {Jung Won} and Lee, {Won Ja}",

note = "Funding Information: This work was supported by a grant from the Pathogenic Proteome Management Program of the National Institute of Health, Ministry of Health and Welfare, Republic of Korea (NIH 48004847-300 ). ",

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AU - Lee, Myoung Ro

AU - Kim, Yu Jung

AU - Kim, Dae Won

AU - Yoo, Won Gi

AU - Cho, Shin Hyeong

AU - Hwang, Kwang Yeon

AU - Ju, Jung Won

AU - Lee, Won Ja

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N2 - Clonorchis sinensis, the causative agent of clonorchiasis, is widespread in East and Southeast Asia, including China, Vietnam and the Republic of Korea. We identified antigenic proteins from adult C. sinensis liver flukes using immunoproteomic analysis. In this study, we found 23 candidate antigenic proteins with a pI in the range of 5.4-6.2 in total lysates of C. sinensis. The antigenic protein spots reacted against sera from clonorchiasis patients and were identified as cysteine proteases, glutathione transferases, gelsolin, propionyl-CoA carboxylase (PCC), prohibitin and 14-3-3 protein (14-3-3) using LC-coupled ESI-MS/MS and an EST database for C. sinensis. PCC and 14-3-3 were identified for the first time as serological antigens for the diagnosis of C. sinensis. To validate the antigenicity of PCC and 14-3-3, recombinant proteins were immunoblotted with sera from clonorchiasis patients. The structural, functional and immunological characteristics of the putative amino acid sequence were predicted by bioinformatics analysis. Our novel finding will contribute to the development of diagnostics for clonorchiasis. These results suggest that immunoproteomic approaches are valuable tools to identify antigens that could be used as targets for effective parasitic infection control strategies.

AB - Clonorchis sinensis, the causative agent of clonorchiasis, is widespread in East and Southeast Asia, including China, Vietnam and the Republic of Korea. We identified antigenic proteins from adult C. sinensis liver flukes using immunoproteomic analysis. In this study, we found 23 candidate antigenic proteins with a pI in the range of 5.4-6.2 in total lysates of C. sinensis. The antigenic protein spots reacted against sera from clonorchiasis patients and were identified as cysteine proteases, glutathione transferases, gelsolin, propionyl-CoA carboxylase (PCC), prohibitin and 14-3-3 protein (14-3-3) using LC-coupled ESI-MS/MS and an EST database for C. sinensis. PCC and 14-3-3 were identified for the first time as serological antigens for the diagnosis of C. sinensis. To validate the antigenicity of PCC and 14-3-3, recombinant proteins were immunoblotted with sera from clonorchiasis patients. The structural, functional and immunological characteristics of the putative amino acid sequence were predicted by bioinformatics analysis. Our novel finding will contribute to the development of diagnostics for clonorchiasis. These results suggest that immunoproteomic approaches are valuable tools to identify antigens that could be used as targets for effective parasitic infection control strategies.

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The identification of antigenic proteins: 14-3-3 protein and propionyl-CoA carboxylase in Clonorchis sinensis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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