Abstract
The objective of this study is to improve cephalosporin C (CPC) production by optimization of medium and culture conditions. A statistical method was introduced to optimize the main culture medium. The main medium for CPC production was optimized using a statistical method. Glucose and corn steep liquor (CSL) were found to be the most effective factors for CPC production. Glucose and CSL were optimized to 2.84 and 6.68%, respectively. CPC production was improved 50% by feeding of 5% rice oil at day 3rd and 5th day during the shake flask culture of C. acremonium M25. The effect of agitation speeds on CPC production in a 2.5-L bioreactor was also investigated with fed-batch mode. The maximum cell mass (54.5 g/L) was obtained at 600 rpm. However, the maximum CPC production (0.98 g/L) was obtained at 500 rpm. At this condition, the maximum CPC production was improved about 132% compared to the result with batch flask culture.
| Original language | English |
|---|---|
| Pages (from-to) | 459-464 |
| Number of pages | 6 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume | 9 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2004 |
Bibliographical note
Funding Information:Acknowledgements This study was supported by reserach grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) at Korea University, Seoul, Korea.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
Keywords
- Cephalosporin C
- Cephalosporium acremonium
- Optimization
- Rice oil
- Statistical method
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
- Biomedical Engineering