The improvement of cephalosporin C production by fed-batch culture of Cephalosporium acremonium M25 using rice oil

Jin Hee Kim, Jung Soo Lim, Seung Wook Kim

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    The objective of this study is to improve cephalosporin C (CPC) production by optimization of medium and culture conditions. A statistical method was introduced to optimize the main culture medium. The main medium for CPC production was optimized using a statistical method. Glucose and corn steep liquor (CSL) were found to be the most effective factors for CPC production. Glucose and CSL were optimized to 2.84 and 6.68%, respectively. CPC production was improved 50% by feeding of 5% rice oil at day 3rd and 5th day during the shake flask culture of C. acremonium M25. The effect of agitation speeds on CPC production in a 2.5-L bioreactor was also investigated with fed-batch mode. The maximum cell mass (54.5 g/L) was obtained at 600 rpm. However, the maximum CPC production (0.98 g/L) was obtained at 500 rpm. At this condition, the maximum CPC production was improved about 132% compared to the result with batch flask culture.

    Original languageEnglish
    Pages (from-to)459-464
    Number of pages6
    JournalBiotechnology and Bioprocess Engineering
    Volume9
    Issue number6
    DOIs
    Publication statusPublished - 2004

    Bibliographical note

    Funding Information:
    Acknowledgements This study was supported by reserach grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) at Korea University, Seoul, Korea.

    Copyright:
    Copyright 2018 Elsevier B.V., All rights reserved.

    Keywords

    • Cephalosporin C
    • Cephalosporium acremonium
    • Optimization
    • Rice oil
    • Statistical method

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology
    • Biomedical Engineering

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