The interaction between E-tropomodulin and thymosin β-10 rescues tumor cells from thymosin β-10 mediated apoptosis by restoring actin architecture

Seung Bae Rho, Taehoon Chun, Seung Hoon Lee, Kyoungsook Park, Je Ho Lee

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Thymosin β-10 (TB10) is a small G-actin binding protein that induces depolymerization of intracellular F-actin pools by sequestering actin monomers. Previously, we demonstrated that overexpression of TB10 in ovarian tumor cells increased the rate of cell death. As an initial step to define molecular mechanism of TB10-dependent apoptotic process in ovarian tumor cells, we searched a human ovary cDNA library for a novel TB10 binding protein using a yeast two-hybrid system. The selected protein was human E-tropomodulin (E-Tmod), another component of the actin binding proteins. Subsequently, two interacting protein components were determined quantitatively. Results showed that the full-length TB10 is required to bind with E-Tmod, and the TB10 binding site on E-Tmod partially overlaps with the actin binding site on E-Tmod. Moreover, introduction of E-Tmod cDNA into a tumor cell line reversed TB10 mediated apoptosis and restored actin architectures. These results may suggest that TB10 regulates apoptotic homeostasis by not only just binding to actin but also competing or blocking the protein complex formation of E-Tmod with actin.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalFEBS Letters
Volume557
Issue number1-3
DOIs
Publication statusPublished - 2004 Jan 16
Externally publishedYes

Bibliographical note

Funding Information:
We thank Drs. S.A. Martinis and S.R. Blanke at the University of Houston for critical reading of the manuscript. This research was supported by Science Research Center (Molecular Therapy Research Center) grant from the Korea Science and Engineering Foundation.

Keywords

  • Actin sequestering protein
  • Apoptosis
  • E-tropomodulin
  • Thymosin β-10
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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