The isoflavones and extracts from Maclura tricuspidata fruit protect against neuronal cell death in ischemic injury via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a

Sungeun Hong; Jaeyoung Kwon; Nguyen Tuan Hiep; Su Jin Sim; Nahyun Kim; Kyeong Ho Kim; Dongho Lee; Woongchon Mar

doi:10.1016/j.jff.2017.12.011

The isoflavones and extracts from Maclura tricuspidata fruit protect against neuronal cell death in ischemic injury via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a

Sungeun Hong, Jaeyoung Kwon, Nguyen Tuan Hiep, Su Jin Sim, Nahyun Kim, Kyeong Ho Kim, Dongho Lee, Woongchon Mar

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

This study evaluated the neuroprotective effects 50% EtOH extract of Maclura tricuspidata fruits (FME50) and isolated compounds, cudraisoflavone I (CFI), and cudraisoflavone H (CFH), on ischemic damage in in vitro model and in vivo model of cerebral ischemia. FME50, CFI, and CFH inhibited OGD/R + glucose-induced neuronal cell death, ROS generation, and Nox4 expression via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a in SH-SY5Y cells. Also, FME50 suppressed OGD/R + glucose-induced activation of ASK1-JNK1/p38 MAPK signal cascade. Furthermore, FME50 significantly reduced the MCAO/R-induced brain infarct, Nox4 expression via induction of Nox4-targeting three miRNAs. Additionally, FME50 suppressed MCAO/R-induced MAPK signal pathway. These results demonstrate that FME50, CFI, and CFH exert neuroprotective effects via Nox4 inhibition by the induction of Nox4-targeting miRNAs and inhibition of MAPK signal cascade, suggesting that they might be possible candidates for the treatment of cerebral ischemia.

Original language	English
Pages (from-to)	785-797
Number of pages	13
Journal	Journal of Functional Foods
Volume	40
DOIs	https://doi.org/10.1016/j.jff.2017.12.011
Publication status	Published - 2018 Jan

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea (Grant no. NRF-2015R1A1A01056603 and NRF-2015R1D1A1A01060321 ) and the BK21 plus program in 2016 through the National Research Foundation (NRF) funded by the Ministry of Education of Korea . The author appreciates Korea University and the Korea Forest Research Institute for supporting the plant materials.

Publisher Copyright:
© 2017 Elsevier Ltd

Keywords

MAPkinase
Maclura tricuspidata
Middle cerebral artery occlusion/reperfusion
MiroRNAs
NADPH oxidase
Oxygen-glucose deprivation/reoxygenation + glucose

ASJC Scopus subject areas

Food Science
Medicine (miscellaneous)
Nutrition and Dietetics

Access to Document

10.1016/j.jff.2017.12.011

Cite this

The isoflavones and extracts from Maclura tricuspidata fruit protect against neuronal cell death in ischemic injury via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a. / Hong, Sungeun; Kwon, Jaeyoung; Hiep, Nguyen Tuan et al.
In: Journal of Functional Foods, Vol. 40, 01.2018, p. 785-797.

Research output: Contribution to journal › Article › peer-review

@article{0aef90661247493daf78e5fa03c9e578,

title = "The isoflavones and extracts from Maclura tricuspidata fruit protect against neuronal cell death in ischemic injury via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a",

abstract = "This study evaluated the neuroprotective effects 50% EtOH extract of Maclura tricuspidata fruits (FME50) and isolated compounds, cudraisoflavone I (CFI), and cudraisoflavone H (CFH), on ischemic damage in in vitro model and in vivo model of cerebral ischemia. FME50, CFI, and CFH inhibited OGD/R + glucose-induced neuronal cell death, ROS generation, and Nox4 expression via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a in SH-SY5Y cells. Also, FME50 suppressed OGD/R + glucose-induced activation of ASK1-JNK1/p38 MAPK signal cascade. Furthermore, FME50 significantly reduced the MCAO/R-induced brain infarct, Nox4 expression via induction of Nox4-targeting three miRNAs. Additionally, FME50 suppressed MCAO/R-induced MAPK signal pathway. These results demonstrate that FME50, CFI, and CFH exert neuroprotective effects via Nox4 inhibition by the induction of Nox4-targeting miRNAs and inhibition of MAPK signal cascade, suggesting that they might be possible candidates for the treatment of cerebral ischemia.",

keywords = "MAPkinase, Maclura tricuspidata, Middle cerebral artery occlusion/reperfusion, MiroRNAs, NADPH oxidase, Oxygen-glucose deprivation/reoxygenation + glucose",

author = "Sungeun Hong and Jaeyoung Kwon and Hiep, {Nguyen Tuan} and Sim, {Su Jin} and Nahyun Kim and Kim, {Kyeong Ho} and Dongho Lee and Woongchon Mar",

note = "Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea (Grant no. NRF-2015R1A1A01056603 and NRF-2015R1D1A1A01060321 ) and the BK21 plus program in 2016 through the National Research Foundation (NRF) funded by the Ministry of Education of Korea . The author appreciates Korea University and the Korea Forest Research Institute for supporting the plant materials. Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2018",

month = jan,

doi = "10.1016/j.jff.2017.12.011",

language = "English",

volume = "40",

pages = "785--797",

journal = "Journal of Functional Foods",

issn = "1756-4646",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - The isoflavones and extracts from Maclura tricuspidata fruit protect against neuronal cell death in ischemic injury via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a

AU - Hong, Sungeun

AU - Kwon, Jaeyoung

AU - Hiep, Nguyen Tuan

AU - Sim, Su Jin

AU - Kim, Nahyun

AU - Kim, Kyeong Ho

AU - Lee, Dongho

AU - Mar, Woongchon

N1 - Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea (Grant no. NRF-2015R1A1A01056603 and NRF-2015R1D1A1A01060321 ) and the BK21 plus program in 2016 through the National Research Foundation (NRF) funded by the Ministry of Education of Korea . The author appreciates Korea University and the Korea Forest Research Institute for supporting the plant materials. Publisher Copyright: © 2017 Elsevier Ltd

PY - 2018/1

Y1 - 2018/1

N2 - This study evaluated the neuroprotective effects 50% EtOH extract of Maclura tricuspidata fruits (FME50) and isolated compounds, cudraisoflavone I (CFI), and cudraisoflavone H (CFH), on ischemic damage in in vitro model and in vivo model of cerebral ischemia. FME50, CFI, and CFH inhibited OGD/R + glucose-induced neuronal cell death, ROS generation, and Nox4 expression via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a in SH-SY5Y cells. Also, FME50 suppressed OGD/R + glucose-induced activation of ASK1-JNK1/p38 MAPK signal cascade. Furthermore, FME50 significantly reduced the MCAO/R-induced brain infarct, Nox4 expression via induction of Nox4-targeting three miRNAs. Additionally, FME50 suppressed MCAO/R-induced MAPK signal pathway. These results demonstrate that FME50, CFI, and CFH exert neuroprotective effects via Nox4 inhibition by the induction of Nox4-targeting miRNAs and inhibition of MAPK signal cascade, suggesting that they might be possible candidates for the treatment of cerebral ischemia.

AB - This study evaluated the neuroprotective effects 50% EtOH extract of Maclura tricuspidata fruits (FME50) and isolated compounds, cudraisoflavone I (CFI), and cudraisoflavone H (CFH), on ischemic damage in in vitro model and in vivo model of cerebral ischemia. FME50, CFI, and CFH inhibited OGD/R + glucose-induced neuronal cell death, ROS generation, and Nox4 expression via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a in SH-SY5Y cells. Also, FME50 suppressed OGD/R + glucose-induced activation of ASK1-JNK1/p38 MAPK signal cascade. Furthermore, FME50 significantly reduced the MCAO/R-induced brain infarct, Nox4 expression via induction of Nox4-targeting three miRNAs. Additionally, FME50 suppressed MCAO/R-induced MAPK signal pathway. These results demonstrate that FME50, CFI, and CFH exert neuroprotective effects via Nox4 inhibition by the induction of Nox4-targeting miRNAs and inhibition of MAPK signal cascade, suggesting that they might be possible candidates for the treatment of cerebral ischemia.

KW - MAPkinase

KW - Maclura tricuspidata

KW - Middle cerebral artery occlusion/reperfusion

KW - MiroRNAs

KW - NADPH oxidase

KW - Oxygen-glucose deprivation/reoxygenation + glucose

UR - http://www.scopus.com/inward/record.url?scp=85037976304&partnerID=8YFLogxK

U2 - 10.1016/j.jff.2017.12.011

DO - 10.1016/j.jff.2017.12.011

M3 - Article

AN - SCOPUS:85037976304

SN - 1756-4646

VL - 40

SP - 785

EP - 797

JO - Journal of Functional Foods

JF - Journal of Functional Foods

ER -

The isoflavones and extracts from Maclura tricuspidata fruit protect against neuronal cell death in ischemic injury via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this