Abstract
This study evaluated the neuroprotective effects 50% EtOH extract of Maclura tricuspidata fruits (FME50) and isolated compounds, cudraisoflavone I (CFI), and cudraisoflavone H (CFH), on ischemic damage in in vitro model and in vivo model of cerebral ischemia. FME50, CFI, and CFH inhibited OGD/R + glucose-induced neuronal cell death, ROS generation, and Nox4 expression via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a in SH-SY5Y cells. Also, FME50 suppressed OGD/R + glucose-induced activation of ASK1-JNK1/p38 MAPK signal cascade. Furthermore, FME50 significantly reduced the MCAO/R-induced brain infarct, Nox4 expression via induction of Nox4-targeting three miRNAs. Additionally, FME50 suppressed MCAO/R-induced MAPK signal pathway. These results demonstrate that FME50, CFI, and CFH exert neuroprotective effects via Nox4 inhibition by the induction of Nox4-targeting miRNAs and inhibition of MAPK signal cascade, suggesting that they might be possible candidates for the treatment of cerebral ischemia.
Original language | English |
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Pages (from-to) | 785-797 |
Number of pages | 13 |
Journal | Journal of Functional Foods |
Volume | 40 |
DOIs | |
Publication status | Published - 2018 Jan |
Bibliographical note
Funding Information:This work was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea (Grant no. NRF-2015R1A1A01056603 and NRF-2015R1D1A1A01060321 ) and the BK21 plus program in 2016 through the National Research Foundation (NRF) funded by the Ministry of Education of Korea . The author appreciates Korea University and the Korea Forest Research Institute for supporting the plant materials.
Publisher Copyright:
© 2017 Elsevier Ltd
Keywords
- MAPkinase
- Maclura tricuspidata
- Middle cerebral artery occlusion/reperfusion
- MiroRNAs
- NADPH oxidase
- Oxygen-glucose deprivation/reoxygenation + glucose
ASJC Scopus subject areas
- Food Science
- Medicine (miscellaneous)
- Nutrition and Dietetics