The isoflavones and extracts from Maclura tricuspidata fruit protect against neuronal cell death in ischemic injury via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a

Sungeun Hong, Jaeyoung Kwon, Nguyen Tuan Hiep, Su Jin Sim, Nahyun Kim, Kyeong Ho Kim, Dongho Lee, Woongchon Mar

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    This study evaluated the neuroprotective effects 50% EtOH extract of Maclura tricuspidata fruits (FME50) and isolated compounds, cudraisoflavone I (CFI), and cudraisoflavone H (CFH), on ischemic damage in in vitro model and in vivo model of cerebral ischemia. FME50, CFI, and CFH inhibited OGD/R + glucose-induced neuronal cell death, ROS generation, and Nox4 expression via induction of Nox4-targeting miRNA-25, miRNA-92a, and miRNA-146a in SH-SY5Y cells. Also, FME50 suppressed OGD/R + glucose-induced activation of ASK1-JNK1/p38 MAPK signal cascade. Furthermore, FME50 significantly reduced the MCAO/R-induced brain infarct, Nox4 expression via induction of Nox4-targeting three miRNAs. Additionally, FME50 suppressed MCAO/R-induced MAPK signal pathway. These results demonstrate that FME50, CFI, and CFH exert neuroprotective effects via Nox4 inhibition by the induction of Nox4-targeting miRNAs and inhibition of MAPK signal cascade, suggesting that they might be possible candidates for the treatment of cerebral ischemia.

    Original languageEnglish
    Pages (from-to)785-797
    Number of pages13
    JournalJournal of Functional Foods
    Volume40
    DOIs
    Publication statusPublished - 2018 Jan

    Bibliographical note

    Funding Information:
    This work was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea (Grant no. NRF-2015R1A1A01056603 and NRF-2015R1D1A1A01060321 ) and the BK21 plus program in 2016 through the National Research Foundation (NRF) funded by the Ministry of Education of Korea . The author appreciates Korea University and the Korea Forest Research Institute for supporting the plant materials.

    Publisher Copyright:
    © 2017 Elsevier Ltd

    Keywords

    • MAPkinase
    • Maclura tricuspidata
    • Middle cerebral artery occlusion/reperfusion
    • MiroRNAs
    • NADPH oxidase
    • Oxygen-glucose deprivation/reoxygenation + glucose

    ASJC Scopus subject areas

    • Food Science
    • Medicine (miscellaneous)
    • Nutrition and Dietetics

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