The multilayer nanoparticles for deep penetration of docetaxel into tumor parenchyma to overcome tumor microenvironment

Nisar Ul Khaliq, Dal Yong Park, Jae Young Lee, Yeonhee Joo, Keun Sang Oh, Jung Seok Kim, Jin Seok Kim, In San Kim, Ick Chan Kwon, Soon Hong Yuk

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Deep penetration of the anticancer drug, docetaxel (DTX), into tumor parenchyma was demonstrated to achieve improved chemotherapy. For this purpose, a multistage nanostructure was designed and characterized using the multilayer nanoparticles (NPs). The multilayer NPs had a core/shell structure. The core was composed of the DTX-loaded Pluronic NPs (diameter: 12 nm) that were transferred into the inner side of vesicles to form the vesicle NPs. Förster resonance energy transfer (FRET) in the NPs was observed to verify the incorporation of the DTX-loaded Pluronic NPs into the inner side of the vesicles during the formation of the vesicle NPs. Subsequently, the vesicle NPs were stabilized through Pluronic-lipid bilayer interaction to form the multilayer NPs. To examine the morphology and size distribution of the multilayer NPs, transmittance electron microscopy and dynamic light scattering were used. In vitro release behavior and toxicity were observed to verify the functionality of the multilayer NPs as nanocarriers for cancer therapy. Multistage functionality was evaluated by cellular uptake and tissue distribution behaviors of the multilayer NPs. The biodistribution of the multilayer NPs and their antitumor efficacy were also observed to understand the role of multistage functionality for improved chemotherapy.

Original languageEnglish
Pages (from-to)833-840
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Publication statusPublished - 2016 Oct 1

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korean government (MEST) ( 20110027932 and 2012028831 ) and National R&D Program for Cancer Control , Ministry of Health and Welfare, Republic of Korea ( 1420390 ).

Publisher Copyright:
© 2016 Elsevier B.V.


  • Chemotherapy
  • Deep penetration
  • Docetaxel
  • Multistage nanostructure
  • The multilayer nanoparticles

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry


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