The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans

M. D. Nelson; N. F. Trojanowski; J. B. George-Raizen; C. J. Smith; C. C. Yu; C. Fang-Yen; D. M. Raizen

doi:10.1038/ncomms3846

The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans

M. D. Nelson, N. F. Trojanowski, J. B. George-Raizen, C. J. Smith, C. C. Yu, C. Fang-Yen, D. M. Raizen

College of Science

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Neuropeptides have central roles in the regulation of homoeostatic behaviours such as sleep and feeding. Caenorhabditis elegans displays sleep-like quiescence of locomotion and feeding during a larval transition stage called lethargus and feeds during active larval and adult stages. Here we show that the neuropeptide NLP-22 is a regulator of Caenorhabditis elegans sleep-like quiescence observed during lethargus. nlp-22 shows cyclical mRNA expression in synchrony with lethargus; it is regulated by LIN-42, an orthologue of the core circadian protein PERIOD; and it is expressed solely in the two RIA interneurons. nlp-22 and the RIA interneurons are required for normal lethargus quiescence, and forced expression of nlp-22 during active stages causes anachronistic locomotion and feeding quiescence. Optogenetic stimulation of the RIA interneurons has a movement-promoting effect, demonstrating functional complexity in a single-neuron type. Our work defines a quiescence-regulating role for NLP-22 and expands our knowledge of the neural circuitry controlling Caenorhabditis elegans behavioural quiescence.

Original language	English
Article number	2846
Journal	Nature communications
Volume	4
DOIs	https://doi.org/10.1038/ncomms3846
Publication status	Published - 2013 Dec 4

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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title = "The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans",

abstract = "Neuropeptides have central roles in the regulation of homoeostatic behaviours such as sleep and feeding. Caenorhabditis elegans displays sleep-like quiescence of locomotion and feeding during a larval transition stage called lethargus and feeds during active larval and adult stages. Here we show that the neuropeptide NLP-22 is a regulator of Caenorhabditis elegans sleep-like quiescence observed during lethargus. nlp-22 shows cyclical mRNA expression in synchrony with lethargus; it is regulated by LIN-42, an orthologue of the core circadian protein PERIOD; and it is expressed solely in the two RIA interneurons. nlp-22 and the RIA interneurons are required for normal lethargus quiescence, and forced expression of nlp-22 during active stages causes anachronistic locomotion and feeding quiescence. Optogenetic stimulation of the RIA interneurons has a movement-promoting effect, demonstrating functional complexity in a single-neuron type. Our work defines a quiescence-regulating role for NLP-22 and expands our knowledge of the neural circuitry controlling Caenorhabditis elegans behavioural quiescence.",

author = "Nelson, {M. D.} and Trojanowski, {N. F.} and George-Raizen, {J. B.} and Smith, {C. J.} and Yu, {C. C.} and C. Fang-Yen and Raizen, {D. M.}",

note = "Funding Information: We thank Amita Sehgal, Liliane Schoofs and Lotte Frooninckx for comments. This work was supported by NIH grants T32HL07713 to M.D.N. (PI, Allan Pack), T31HL07953 to N.F.T. (PI, Allan Pack), R01NS064030 to D.M.R., a NARSAD Young Investigator Award to D.M.R., a McCabe Foundation grant to D.M.R., a Penn University Scholars Award to C.-C.Y. and by an Alfred P. Sloan Foundation Research Fellowship to C.F.-Y. Some strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). The strain ARF240 was provided by A. Frand and the strain VM1345 was provided by A. Maricq.",

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doi = "10.1038/ncomms3846",

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T1 - The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans

AU - Nelson, M. D.

AU - Trojanowski, N. F.

AU - George-Raizen, J. B.

AU - Smith, C. J.

AU - Yu, C. C.

AU - Fang-Yen, C.

AU - Raizen, D. M.

N1 - Funding Information: We thank Amita Sehgal, Liliane Schoofs and Lotte Frooninckx for comments. This work was supported by NIH grants T32HL07713 to M.D.N. (PI, Allan Pack), T31HL07953 to N.F.T. (PI, Allan Pack), R01NS064030 to D.M.R., a NARSAD Young Investigator Award to D.M.R., a McCabe Foundation grant to D.M.R., a Penn University Scholars Award to C.-C.Y. and by an Alfred P. Sloan Foundation Research Fellowship to C.F.-Y. Some strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). The strain ARF240 was provided by A. Frand and the strain VM1345 was provided by A. Maricq.

PY - 2013/12/4

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N2 - Neuropeptides have central roles in the regulation of homoeostatic behaviours such as sleep and feeding. Caenorhabditis elegans displays sleep-like quiescence of locomotion and feeding during a larval transition stage called lethargus and feeds during active larval and adult stages. Here we show that the neuropeptide NLP-22 is a regulator of Caenorhabditis elegans sleep-like quiescence observed during lethargus. nlp-22 shows cyclical mRNA expression in synchrony with lethargus; it is regulated by LIN-42, an orthologue of the core circadian protein PERIOD; and it is expressed solely in the two RIA interneurons. nlp-22 and the RIA interneurons are required for normal lethargus quiescence, and forced expression of nlp-22 during active stages causes anachronistic locomotion and feeding quiescence. Optogenetic stimulation of the RIA interneurons has a movement-promoting effect, demonstrating functional complexity in a single-neuron type. Our work defines a quiescence-regulating role for NLP-22 and expands our knowledge of the neural circuitry controlling Caenorhabditis elegans behavioural quiescence.

AB - Neuropeptides have central roles in the regulation of homoeostatic behaviours such as sleep and feeding. Caenorhabditis elegans displays sleep-like quiescence of locomotion and feeding during a larval transition stage called lethargus and feeds during active larval and adult stages. Here we show that the neuropeptide NLP-22 is a regulator of Caenorhabditis elegans sleep-like quiescence observed during lethargus. nlp-22 shows cyclical mRNA expression in synchrony with lethargus; it is regulated by LIN-42, an orthologue of the core circadian protein PERIOD; and it is expressed solely in the two RIA interneurons. nlp-22 and the RIA interneurons are required for normal lethargus quiescence, and forced expression of nlp-22 during active stages causes anachronistic locomotion and feeding quiescence. Optogenetic stimulation of the RIA interneurons has a movement-promoting effect, demonstrating functional complexity in a single-neuron type. Our work defines a quiescence-regulating role for NLP-22 and expands our knowledge of the neural circuitry controlling Caenorhabditis elegans behavioural quiescence.

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JO - Nature Communications

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The neuropeptide NLP-22 regulates a sleep-like state in Caenorhabditis elegans

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